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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

LC-HRMS and acetylcholinesterase affinity assay as a workflow for profiling alkaloids in Annona salzmannii extract

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Author(s):
Lima, Juliana M. [1] ; Leme, Gabriel M. [1] ; Costa, Emmanoel V. [2] ; Cass, Quezia B. [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Carlos, Dept Quim, Separare, Sao Carlos, SP - Brazil
[2] Univ Fed Amazonas, Inst Ciencias Exatas, Dept Quim, Manaus, Amazonas - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES; v. 1164, FEB 1 2021.
Web of Science Citations: 0
Abstract

Structure-based molecular networking is useful as a dereplication strategy to identify known molecules, unknown close analogues, or compound families. On the other hand, the ligand fishing assay is a remarkable alternative to accelerate the screening process and to overcome the drawbacks of laborious experiments usually adopted in natural product research. The combination of these approaches contributes to high productivity in disclosing active metabolites and a decrease in lead time identification. To provide a valuable data base for the alkaloids of A. salzmannii bark herein we disclose thirty-one isoquinoline alkaloids including benzyltetrahydroisoquinolines, aporphines, proaporphines, and protoberberines. Among these, twenty-six have not been described for A. salzmannii including the unprecedented alkaloid N,O-dimethylcoclaurine N-oxide. In addition, norcoclaurine (1), norreticuline (13), N,O-dimethylcoclaurine N-oxide (15), and N-acetylasimilobine (24) are now reported for the first time as ligand for acetylcholinesterase. (AU)

FAPESP's process: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
Grantee:Arlene Gonçalves Corrêa
Support Opportunities: Research Grants - Research Centers in Engineering Program
FAPESP's process: 16/24957-0 - Models of binders rapid screening in extracts of natural products for bioconjugation tests and LC-HRMS
Grantee:Gabriel Mazzi Leme
Support Opportunities: Scholarships in Brazil - Post-Doctoral