Effects of high-intensity interval training in more or less active mice on biomechanical, biophysical and biochemical bone parameters

Author(s)

Polisel, Emanuel E. C. ^[1] ; Beck, Wladimir R. ^[2] ; Scariot, Pedro P. M. ^[1] ; Pejon, Taciane M. M. ^[2] ; Gobatto, Claudio A. ^[1] ; Manchado-Gobatto, Fulvia B. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Appl Sport Physiol, Pedro Zaccaria St 1-300, BR-13484350 Limeira, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Physiol Sci, Lab Endocrine Physiol & Phys Exercise, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 11, n. 1 MAR 19 2021.
• Web of Science Citations: 0

Abstract

High-intensity interval training (HIIT) is of scientific interest due its role in improving physical fitness, but the effects of HIIT on bone health need be carefully explored. Further, it is necessary to know whether HIIT effects on bone health are dependent on the physical activity levels. This may be experimentally tested since we have built a large cage (LC) that allows animals to move freely, promoting an increase of spontaneous physical activity (SPA) in comparison to a small cage (SC). Thus, we examined the effects of HIIT on biophysical, biomechanical and biochemical parameters of bone tissue of C57BL/6J mice living in cages of two different sizes: small (SC) or large (LC) cages with 1320 cm 2 and 4800 cm(2) floor space, respectively. Male mice were subdivided into two groups within each housing type: Control (C) and Trained (T). At the end of the interventions, all mice were euthanized to extract the femur bone for biophysical, biomechanical and biochemical analyses. Based a significant interaction from two-way ANOVA, trained mice kept in large cage (but not for trained mice housed in SC) exhibited a reduction of tenacity and displacement at failure in bone. This suggests that long-term HIIT program, in addition with a more active lifestyle correlates with exerts negative effects on the bone of healthy mice. A caution must also be raised about the excessive adoption of physical training, at least regarding bone tissue. On the other hand, increased calcium was found in femur of mice housed in LC. In line with this, LC-C mice were more active (i.e. SPA) than other groups. This implies that an active lifestyle without long-term high intensity physical training seems to play a role in promoting benefits to bone tissue. Our data provides new insights for treatment of osteo-health related disorders. (AU)

• FAPESP's process: 15/00272-6 - Effects of caloric restriction mediated by SIRT3 in the aerobic and anaerobic capacities of mice: relations with the spontaneous activity, metabolic parameters and muscle mitochondrial function in aging process
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 18/07099-6 - High intensity interval training (HIIT) in running: effects on aerobic and anaerobic capacity, stress biomarkers, bone and muscle responses in active and sedentary mice
• Grantee: Emanuel Elias Camolese Polisel
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/05115-7 - Effects of aerobic training and the hypoxic environment on molecular responses related to anaerobic and aerobic metabolism and acid-base properties in the hypothalamus and skeletal muscle of mice
• Grantee: Pedro Paulo Menezes Scariot
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/01362-9 - Effects of age, caloric restriction diet (Cr) and SIRT3 on spontaneous activity, behavior and oxidative mediators (NO, NOS and orexin) and hypothalamic mitochondrial respiration
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 19/08148-3 - Melatonin and physical performance: from ergogenic to accelerated skeletal muscle repair after damage induced by physical exercise
• Grantee: Wladimir Rafael Beck
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/10336-9 - Effect of different training models with load control over physiological parameters, genic expressions and protein contents of HIF-1 alpha, PGC-1 alpha, MCT1 and MCT4: relationship with performance and spontaneous activity of swimming rats
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/10201-4 - Effects of aerobic physical training and the availability of wide space housing on the spontaneous physical activity, central and peripheral metabolic and molecular responses
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Regular Research Grants