Twenty-one days of low-intensity eccentric training improve morphological characteristics and function of soleus muscles of mdx mice

Author(s)

Pedrazzani, Paulo S. ^[1] ; Araujo, Tatiana O. P. ^[1] ; Sigoli, Emilly ^[1] ; da Silva, Isabella R. ^[1] ; da Roza, Daiane Leite ^[2] ; Chesca, Deise Lucia ^[3] ; Rassier, Dilson E. ^[4] ; Cornachione, Anabelle S. ^[1]

Affiliation

^[1] Fed Univ Sao Carlos UFSCar, Dept Physiol Sci, Sao Carlos - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Neurosci & Behav, Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Legal Med, Ribeirao Preto - Brazil

^[4] McGill Univ, Dept Kinesiol & Phys Educ, Montreal, PQ - Canada

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 11, n. 1 FEB 11 2021.
• Web of Science Citations: 0

Abstract

Duchene muscular dystrophy (DMD) is caused by the absence of the protein dystrophin, which leads to muscle weakness, progressive degeneration, and eventually death due to respiratory failure. Low-intensity eccentric training (LIET) has been used as a rehabilitation method in skeletal muscles after disuse. Recently, LIET has also been used for rehabilitating dystrophic muscles, but its effects are still unclear. The purpose of this study was to investigate the effects of 21 days of LIET in dystrophic soleus muscle. Thirty-six male mdx mice were randomized into six groups (n=6/each): mdx sedentary group; mdx training group-3 days; mdx training group-21 days; wild-type sedentary group; wild-type training group-3 days and wild-type training group-21 days. After the training sessions, animals were euthanized, and fragments of soleus muscles were removed for immunofluorescence and histological analyses, and measurements of active force and Ca2+ sensitivity of the contractile apparatus. Muscles of the mdx training group-21 days showed an improvement in morphological characteristics and an increase of active force when compared to the sedentary mdx group. The results show that LIET can improve the functionality of dystrophic soleus muscle in mice. (AU)

• FAPESP's process: 17/03843-0 - Effects of eccentric exercise on myosin heavy chain (MHC) and creatine kinase (CK) enzyme expression on skeletal muscle of mdx mice
• Grantee: Isabella Ramily da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/07104-6 - Effects of eccentric exercise in skeletal muscle rehabilitation of mdx mice after long period of training: morphological, functional and molecular evaluations
• Grantee: Anabelle Silva Cornachione
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/07581-2 - Mortality in mental disorders with recent onset psychotic manifestations
• Grantee: Daiane Leite da Roza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/00267-8 - Effects of eccentric training on the skeletal muscle of mdx mice after long period of training. morphological and functional evaluations
• Grantee: Paulo Silva Pedrazzani
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/21048-0 - Effects of eccentric exercise on muscle fibers different types and on the contraction force of permeabilized single fibers of dystrophic muscles
• Grantee: Emilly Sigoli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/25634-2 - Effects of eccentric exercise in skeletal muscle rehabilitation of mdx mice after a long period of training: morphological, functional and molecular evaluations
• Grantee: Anabelle Silva Cornachione
• Support Opportunities: Scholarships in Brazil - Young Researchers