Antimicrobial resistance of Staphylococcus spp. isolated from organic and conventional Minas Frescal cheese producers in Sao Paulo, Brazil

Author(s)

da Silva Abreu, Anderson Clayton ^[1] ; Matos, Luiz Gustavo ^[1] ; da Silva Candido, Talita Junia ^[1] ; Barboza, Giovana Rueda ^[1] ; Martins Alencar de Souza, Victoria Vilaca ^[1] ; Munive Nunez, Karen Vanessa ^[1] ; Cirone Silva, Nathalia Cristina ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF DAIRY SCIENCE; v. 104, n. 4, p. 4012-4022, APR 2021.
• Web of Science Citations: 0

Abstract

The genus Staphylococcus is recognized worldwide as a cause of bacterial infections in humans and animals. Antibiotics used in dairy cattle combined with ineffective control can increase antimicrobial resistance. The objective of this study was to characterize 95 Staphylococcus strains isolated from organic and conventional Minas Frescal cheese production regarding antibiotic resistance (phenotype and genotype), presence of sanitizer-resistant genes and biofilm-formation genes, and SCCmec typing. Most strains (25.3%) showed higher resistance to penicillin, followed by oxacillin (21.1%) and clindamycin (11.6%). Among antibiotic resistance genes, the most prevalent were blaZ (25.3%), mecA (13.7%), lsaB (6.3%), msrA (4.2%), ant4 (3.2%), and tetM (2.1%); among sanitizer-resistance genes they were qacA/B (5.3%) and qacC (6.3%); and among biofilm, bap (4.2%), icaA (29.5%), icaD (41.1%). However, there was no statistically significant difference between organic and conventional dairy products, possibly due to the lack of synthetic antibiotic use on conventional farms during the sample collection period. Methicillin-resistant Staphylococcus aureus (MRSA) had their SCCmec identified as types I and IVc, and the methicillin-resistant coagulase-negative staphylococci had nontypeable SCCmec. These results suggest that there are antibiotic-resistant strains in both organic and conventional Minas Frescal cheese production in the state of Sao Paulo, Brazil. This supports the idea that improved quality control is needed from the milking stage up to the final product. (AU)

• FAPESP's process: 16/02118-7 - Molecular biology tools to study microbial diversity and characterization of Staphylococcus aureus in organic and conventional dairy in São Paulo
• Grantee: Nathalia Cristina Cirone Silva
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/00986-7 - Coagulase-negative Staphylococcus: analyze of the resistance profile and enterotoxigenic in strains isolated from dairy products
• Grantee: Luiz Gustavo de Matos
• Support Opportunities: Scholarships in Brazil - Scientific Initiation