Staphylococcus spp. isolated from sub clinic mastitis in different Brazilian States: antimicrobial resistance, detection of virulence factors and identification of clonal profiling

• Grant number: 15/01401-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2015
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Maria de Lourdes Ribeiro de Souza da Cunha
• Grantee: Maria de Lourdes Ribeiro de Souza da Cunha
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Antimicrobials are often used to contribute to increase the selective pressure of microorganisms that are resistant to the main drugs. Animal World Health Organization aims to protect animal and human health against the risk of antimicrobial resistance from sick animals treatments. This study aims to identify and characterize clonal profile, virulence factors and antimicrobial resistance factors in Staphylococcus spp. isolated from cattle milk of animals with subclinical mastitis of different zones in Brasil. The samples will be obtained by "Embrapa Gado de Leite" from six different Brazilian states including Paraná, Santa Catarina, Rio Grande do Sul, Minas Gerais, São Paulo and Pernambuco. This study includes all Staphylococcus spp. strains that shows to be oxacillin resistant by the disc diffusion method. This samples will be phenotypic and genotypic identified followed by an investigation of enterotoxins and biofilm genes encoding presence. Thereafter, the study will include the determination of oxacilin resistance by identifying the mecA and homologue mecA genes (mecC) and the vancomycin resistance screening. Positive isolates to biofilm and toxin genes encoding presence will pass thought the qRT-PCR technique to determinate these virulence factors expression. Furthermore the Staphylococcus spp. clonal profile will be investigated using the Pulsed Field Gel Electrophoresis (PFGE) technique and the Multilocus Sequence Typing (MLST) technique; this last one by typing the prevalent clones. Thus, the staphylococci species clonal profile isolated from cattle milk may contribute to understand the present strains standards on different herds, allowing measures to prevent and control the spread of pathogenic and multiresistant staphylococci clones in different Brazilian zones. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELLO, PRISCILA LUIZA; MORAES RIBOLI, DANILO FLAVIO; MARTINS, LISIANE DE ALMEIDA; VASCONCELOS PAIVA BRITO, MARIA APARECIDA; VICTORIA, CASSIANO; ROMERO, LETICIA CALIXTO; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. Staphylococcus spp. Isolated from Bovine Subclinical Mastitis in Different Regions of Brazil: Molecular Typing and Biofilm Gene Expression Analysis by RT-qPCR. ANTIBIOTICS-BASEL, v. 9, n. 12, DEC 2020. (12/24135-0, 15/01401-4)

MELLO, PRISCILA LUIZA; PINHEIRO, LUIZA; MARTINS, LISIANE DE ALMEIDA; VASCONCELOS PAIVA BRITO, MARIA APARECIDA; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. beta-Lactam resistance and vancomycin heteroresistance in Staphylococcus spp. isolated from bovine subclinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 100, n. 8, p. 6567-6571, AUG 2017. (12/24135-0, 15/01401-4)

MELLO, PRISCILA LUIZA; MORAES RIBOLI, DANILO FLAVIO; PINHEIRO, LUIZA; MARTINS, LISIANE DE ALMEIDA; VASCONCELOS PAIVA BRITO, MARIA APARECIDA; RIBEIRO DE SOUZA DA CUNHA, MARIA DE LOURDES. Detection of Enterotoxigenic Potential and Determination of Clonal Profile in Staphylococcus aureus and Coagulase-Negative Staphylococci Isolated from Bovine Subclinical Mastitis in Different Brazilian States. TOXINS, v. 8, n. 4, APR 2016. (12/24135-0, 15/01401-4)