Nonsteroidal anti-inflammatory drugs act as endocrine disruptors in Astyanax lacustris (Teleostei: Characidae) reproduction: An ex vivo approach

Author(s)

Branco, Giovana Souza ^{[1, 2]} ; Moreira, Renata Guimaraes ^[1] ; Borella, Maria Ines ^[3] ; Camargo, Marilia de Paiva ^[3] ; Munoz-Penuela, Marcela ^[1] ; Gomes, Aline Dal Olio ^[1] ; Tolussi, Carlos Eduardo ^[4]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Lab Metab & Reprod Organismos Aquat, Rua Matao, Trav 14, 321, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, Univ Estadual Paulista CAUNESP, Ctr Aquicultura, V Acesso Prof Paulo Donato Castelane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Lab Endocrinol Peixes, Av Prof Lineu Prestes 1524, Lab 426, BR-05508000 Sao Paulo, SP - Brazil

^[4] Univ Anhembi Morumbi, Escola Ciencias Saude, R Dr Almeida Lima 1134, Parque Mooca, BR-03164000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: AQUATIC TOXICOLOGY; v. 232, MAR 2021.
• Web of Science Citations: 0

Abstract

Pharmaceutical products can act as endocrine-disrupting compounds (EDCs), affecting the physiological processes of animals, such as development or reproduction. This study aimed to investigate the influence of different concentrations of nonsteroidal anti-inflammatory drugs (NSAIDs) diclofenac (DCF) and ibuprofen (IBU) alone and mixed (MIX) on gonadotmpin gene expression and gonadal steroid release using Astyanax lacustris pituitary and testes explant systems, respectively. The explant organs were maintained for 12 h in Leibovitz (L-15) medium supplemented with 0, 0.1, 1, 10, 100, and 1000 ng L-1 of DCF, IBU, and MIX (ratio 1:1 of the same concentrations of DCF and IBU alone) and gonadotropin releasing-hormone (cGnRH2) stimulation in pituitary explants and human chorionic gonadotmpin (hCG) stimulation in testes explants. The pituitary glands and the media from the testicular explants were collected for gene expression analysis including the beta subunit of the follicle-stimulating hormone (fsh beta) and luteinizing hormone (Ih beta) and secreted gonadal steroid concentration analysis, respectively. Both DCF and IBU (alone and mixed) decreased pituitary gene expression of fsh beta and Ih beta and this inhibitory effect was evident even at low concentrations. In the testes, DCF and IBU did not change the levels of estradiol, and both pharmaceuticals increased the release of 11-ketotestosterone at low doses, while only IBU decreased the levels of testosterone in all concentrations. IBU' s inhibitory effect in the testes was not triggered by the mixture of the two drugs. These results suggest that NSAIDs, may interfere in fish reproduction by acting as EDCs, thereby negatively affecting A. lacustris spermatogenesis and maturation. (AU)

• FAPESP's process: 17/11530-1 - Physiological adjustments of tropical teleosts in anthropogenic reservoirs
• Grantee: Renata Guimarães Moreira Whitton
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/16320-7 - Impacts of climate/environmental change on the fauna: an integrative approach
• Grantee: Carlos Arturo Navas Iannini
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants