The plasmidome of multidrug-resistant emergent Salmonella serovars isolated from poultry

Author(s)

Galetti, Renata ^[1] ; Casarin Penha Filho, Rafael Antonio ^[2] ; Ferreira, Joseane Cristina ^[1] ; Varani, Alessandro M. ^[2] ; Sazinas, Pavelas ^[3] ; Jelsbak, Lars ^[3] ; Costa Darini, Ana Lucia ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Fammceut Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto - Brazil

^[2] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Jaboticabal - Brazil

^[3] Tech Univ Denmark, Lyngby - Denmark

• Document type: Journal article
• Source: INFECTION GENETICS AND EVOLUTION; v. 89, APR 2021.
• Web of Science Citations: 0

Abstract

The rapid emergence of resistant bacteria is occurring worldwide. The understanding of the dissemination of antimicrobial resistance using high-throughput sequencing and bioinformatics approaches is providing valuable insights into the genetic basis of the horizontal gene transfer and the emergence of the antibiotic resistance threat. This ultimately can offer vital clues to the development of coordinated efforts to implement new policies to continue fighting against bacterial infections. The poultry microbiota is characterized as a potential reservoir of resistance genes, mostly derived from the Enterobacteriaceae which have become increasingly important in human and animal infections. In this work, complete genome sequences were achieved for four multidrugresistant Salmonella spp. isolated from poultry from different farms in Brazil. We identified highly similar IncHI2-ST2 megaplasmids (larger than 275.000 bp) in all Salmonella isolates studied. These megaplasmids carry a resistome comprised of eleven different resistance genes (aac(6?)-Iaa, aadA1b, aph(4)-Ia, aph(6)-Id, aph(3?)-Ib, aph(3?)-Ia, aac(3)-Iva, sul1, tetA, tetB and dfrA1b) and four heavy metal tolerance operons (telluride, mercury, silver and copper). In conclusion, the multidrug-resistant plasmids identified in S. enterica serovar Schwarzengrund and Newport isolated from poultry show a variety of antibiotic resistance and heavy metal tolerance genes, providing advantages for the bacteria to survive under extremely unfavorable conditions. (AU)

• FAPESP's process: 15/11728-0 - Complete sequencing of plasmids carrying antibiotic resistance genes in bacteria of interest and genome analysis of Pseudomonas aeruginosa-producing SPM-1 and KPC-2 lines by large-scale sequencing
• Grantee: Renata Galetti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/26311-3 - High-throughput genome sequencing of Enterobacteriaceae resistance plasmids isolated from poultry
• Grantee: Renata Galetti
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 14/14494-8 - Molecular epidemiology of gram-negative bacteria and genetic of antibiotic resistance
• Grantee: Ana Lúcia da Costa Darini
• Support Opportunities: Research Projects - Thematic Grants