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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Fc gamma receptors on aging neutrophils

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Author(s):
Gasparoto, Thais Helena [1] ; Dalboni, Thalita Marcato [1] ; Amor, Nadia Ghinelli [1] ; Abe, Aneli Eiko [1] ; Perri, Graziela [1] ; Lara, Vanessa Soares [2] ; Vieira, Narciso Almeida [3] ; Gasparoto, Carlos Teodoro [4] ; Campanelli, Ana Paula [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Odontol Bauru, Dept Ciencias Biol, Bauru, SP - Brazil
[2] Univ Sao Paulo, Fac Odontol Bauru, Dept Estomatol Patol Oral, Bauru, SP - Brazil
[3] Hosp Anomalies Craniofaciais HRAC, Bauru, SP - Brazil
[4] Univ Sao Paulo, Fac Med Sao Paulo, Dept Saude Publ, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Applied Oral Science; v. 29, 2021.
Web of Science Citations: 0
Abstract

Objective: Neutrophils are key effector cells of the innate immune system. They recognize antigens through membrane receptors, which are expressed during their maturation and activation. Neutrophils express Fc gamma RII (CD32), Fc gamma RIII (CD16), and Fc gamma RI (CD64) after being activated by different factors such as cytokines and bacterial products. These receptors are involved with phagocytosis of IgG-opsonized microbes and enhance defense mechanisms. Based on that, our study seeks to compare the expression of Fc gamma RII, Fc gamma RIII, Fc gamma RI, and CD11b on neutrophils from elderly and young subjects and their expression after in vitro activation with cytokines and LPS. Methodology: Neutrophils were isolated from human peripheral blood and from mice bone marrow by density gradient. After isolation, FC gamma Rs expression was immediately analyzed by flow cytometry or after in vitro stimulation. Results: In freshly isolated cells, the percentage of Fc gamma RIIIb(+) and CD11b(+) neutrophils were higher in samples from young individuals; Fc gamma RIIIa expression was more prominent on aged neutrophils; Fc gamma RIA expression was similar in all samples analyzed. Exposure to CXCL8 and LPS resulted in a higher percentage of Fc gamma RIa(+) neutrophils on elderly individuals' samples but lower when compared with neutrophils from young donors. We observed that LPS caused an increase in Fc gamma RIIa expression on aging human neutrophils. In contrast, Fc gamma RIIIb expression in response to CXCL8 and LPS stimulation was not altered in the four groups. CD11b expression was lower in neutrophils from elderly individuals even in response to LPS and CXCL8. In mice, we observed differences only regarding CD11b expression, which was increased on aged neutrophils. LPS exposure caused an increase in all Fc gamma Rs. Conclusions: Our results suggest that, in humans, the overall pattern of Fc gamma R expression and integrin CD11b are altered during aging and immunosenescence might contribute to age-related infection. (AU)

FAPESP's process: 09/14127-7 - The role of inflammasomes in chemically induced tumor
Grantee:Thaís Helena Gasparoto
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/19317-6 - Activation profile of neutrophils from elderly individuals and expression of IgG receptors
Grantee:Thalita Marcato Dalboni
Support Opportunities: Scholarships in Brazil - Scientific Initiation