Post-resistance exercise photobiomodulation therapy has a more effective antioxidant effect than pre-application on muscle oxidative stress

Author(s)

Sunemi, Simone Mitie ^[1] ; Teixeira, Ighor Luiz Azevedo ^[2] ; Mansano, Barbara Sampaio Dias Martins ^[1] ; de Oliveira, Helenita Antonia ^[2] ; Antonio, Ednei Luiz ^[2] ; de Souza Oliveira, Claudia ^[1] ; Leal-Junior, Ernesto Cesar Pinto ^[1] ; Tucci, Paulo Jose Ferreira ^[2] ; Serra, Andrey Jorge ^{[1, 2, 3]}

Affiliation

^[1] Univ Nove Julho, Programa Posgrad Biofoton Aplicada Ciencias Saude, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Programa Posgrad Cardiol, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Cardiol Div, Med Dept, Rua Pedro Toledo 781, 10 Andar, Sao Paulo - Brazil

• Document type: Journal article
• Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 20, n. 4 APR 2021.
• Web of Science Citations: 0

Abstract

This study evaluated the effect of photobiomodulation therapy (PBMt) before or after a high-intensity resistance exercise (RE) session on muscle oxidative stress. Female Wistar rats were assigned to one of the following groups: Sham (non-exercised, undergoing placebo-PBMt); NLRE (exercised, undergoing placebo-PBMt); PBMt + RE (pre-exercise PBMt); RE + PBMt (post-exercise PBMt). The RE comprised four climbs bearing the maximum load with a 2 min rest between each climb. An 830-nm aluminum gallium arsenide diode laser (100 mW; 0.028 cm(2); 3.57 mW/cm(2); 142.8 J/cm(2); 4 J; Photon Laser III, DMC, Sao Paulo, Brazil) was applied 60 s before or after RE in gastrocnemius muscles. Analyses were performed at 24 h after RE: lipoperoxidation using malondialdehyde (MDA) and protein oxidation (OP) on Western blot. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activity were spectrophotometrically assessed. Nitric oxide (NO) level was determined by the Griess reaction. The MDA and OP levels were significantly higher in the NLRE group. Increased OP was prevented in all PBMt groups; however, increased MDA was prevented only in the RE + PBMT group. The RE + PBMt group had higher SOD activity compared to all other groups. A higher GPx activity was observed only in the PBMT + RE compared to Sham group, and CAT activity was reduced by RE, without PBMt effect. NO levels were unchanged with RE or PBMt. Therefore, PBMt application after a RE section has a more potent antioxidant effect than previous PBMt. Rats submitted to post-RE PBMt illustrated prevention of increased lipoperoxidation and protein oxidation as well as increased SOD activity. {[}GRAPHICS] . (AU)

• FAPESP's process: 18/06865-7 - Photobiomodulation with light-emitting diode (LED) can improve the efficiency of mesenquimial stem cell therapy in post-ischemic heart failure?
• Grantee: Andrey Jorge Serra
• Support Opportunities: Regular Research Grants