BDNF as a Putative Target for Standardized Extract of Ginkgo biloba-Induced Persistence of Object Recognition Memory

Author(s)

Muratori, Beatriz G. ^[1] ; Zamberlam, Claudia R. ^[1] ; Mendes, Thais B. ^[2] ; Nozima, Bruno H. N. ^[2] ; Cerutti, Janete M. ^[2] ; Cerutti, Suzete M. ^{[3, 1]}

Affiliation

^[1] Univ Fed Sao Paulo, Cellular & Behav Pharmacol Lab, Grad Program Biol Chem, BR-09972270 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Morphol & Genet, Div Genet, Genet Bases Thyroid Tumor Lab, BR-04039032 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Biol Sci, BR-09972270 Sao Paulo - Brazil

• Document type: Journal article
• Source: Molecules; v. 26, n. 11 JUN 2021.
• Web of Science Citations: 0

Abstract

Despite considerable progress on the study of the effect of standardized extract of Gingko biloba (EGb) on memory processes, our understanding of its role in the persistence of long-term memory (LTM) and the molecular mechanism underlying its effect, particularly episodic-like memory, is limited. We here investigated the effects of EGb on the long-term retention of recognition memory and its persistence and BDNF expression levels in the dorsal hippocampal formation (DHF). Adult male Wistar rats (n = 10/group) were handled for 10 min/5 day. On day 6, the animals were treated with vehicle or 0.4 mg/kg diazepam (control groups) or with EGb (250, 500 or 100 mg/kg) 30 min before the training session (TR1), in which the animals were exposed to two sample objects. On day 7, all rats underwent a second training session (TR2) as described in the TR1 but without drug treatment. Object recognition memory (ORM) was evaluated on day 8 (retention test, T1) and day 9 (persistence test, T2). At the end of T1or T2, animals were decapitated, and DHF samples were frozen at -80 degrees C for analyses of the differential expression of BDNF by Western blotting. EGb-treated groups spent more time exploring the novel object in T2 and showed the highest recognition index (RI) values during the T1 and T2, which was associated with upregulation of BDNF expression in the DHF in a dose-and session-dependent manner. Our data reveal, for the first time, that EGb treatment before acquisition of ORM promotes persistence of LTM by BDNF differential expression. (AU)

• FAPESP's process: 16/18039-9 - Participation of the hippocampal formation in acquisition, consolidation, evocation and extinction on conditioned suppression: clinical correlations
• Grantee: Suzete Maria Cerutti
• Support Opportunities: Regular Research Grants