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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Hydroxyquinol Degradation Pathway in Rhodococcus jostii RHA1 and Agrobacterium Species Is an Alternative Pathway for Degradation of Protocatechuic Acid and Lignin Fragments

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Author(s):
Spence, Edward M. [1] ; Scott, Heather T. [1] ; Dumond, Louison [1] ; Calvo-Bado, Leonides [1] ; di Monaco, Sabrina [1] ; Williamson, James J. [1] ; Persinoti, Gabriela F. [2] ; Squina, Fabio M. [3] ; Bugg, Timothy D. H. [1]
Total Authors: 9
Affiliation:
[1] Univ Warwick, Dept Chem, Coventry, W Midlands - England
[2] Lab Nacl Biorrenovaveis LNBR CNPEM, Campinas - Brazil
[3] Univ Sorocaba, Programa Proc Tecnol & Ambientais, Sorocaba - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Applied and Environmental Microbiology; v. 86, n. 19 OCT 2020.
Web of Science Citations: 3
Abstract

Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on medium containing vanillin or protocatechuic acid indicated the formation of hydroxyquinol (benzene-1,2,4-triol) as a downstream product. Gene cluster ro01857-ro01860 in Rhodococcus jostii RHA1 contains genes encoding hydroxyquinol 1,2-dioxygenase and maleylacetate reductase for degradation of hydroxyquinol but also putative mono-oxygenase (ro01860) and putative decarboxylase (ro01859) genes, and a similar gene cluster is found in the genome of lignin-degrading Agrobacterium species. Recombinant R. jostii mono-oxygenase and decarboxylase enzymes in combination were found to convert protocatechuic acid to hydroxyquinol. Hence, an alternative pathway for degradation of protocatechuic acid via oxidative decarboxylation to hydroxyquinol is proposed. IMPORTANCE There is a well-established paradigm for degradation of protocatechuic acid via the beta-ketoadipate pathway in a range of soil bacteria. In this study, we have found the existence of a second pathway for degradation of protocatechuic acid in Rhodococcus jostii RHA1, via hydroxyquinol (benzene-1,2,4-triol), which establishes a metabolic link between protocatechuic acid and hydroxyquinol. The presence of this pathway in a lignin-degrading Agrobacterium sp. strain suggests the involvement of the hydroxyquinol pathway in the metabolism of degraded lignin fragments. (AU)

FAPESP's process: 15/50590-4 - Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals
Grantee:Fábio Márcio Squina
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants