Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Moniliophthora perniciosa, the causal agent of witches' broom disease of cacao, interferes with cytokinin metabolism during infection of Micro-Tom tomato and promotes symptom development

Full text
Author(s):
Show less -
Costa, Juliana L. [1] ; Paschoal, Daniele [1, 2] ; da Silva, Eder M. [1] ; Silva, Jamille S. [1, 2] ; do Carmo, Rafael M. [1] ; Carrera, Esther [3] ; Lopez-Diaz, Isabel [3] ; Rossi, Monica L. [1] ; Freschi, Luciano [4] ; Mieczkowski, Piotr [5] ; Peres, Lazaro E. P. [2] ; Teixeira, Paulo J. P. L. [5, 2] ; Figueira, Antonio [1]
Total Authors: 13
Affiliation:
[1] Univ Sao Paulo, Ctr Energia Nucl Agr, Ave Centenario 303, BR-13400970 Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 9, BR-13418900 Piracicaba, SP - Brazil
[3] Univ Politecn Valencia UPV, CSIC, Inst Biol Mol & Celular Plantas, Ingeniero Fausto Elio S-N, Valencia 46022 - Spain
[4] Univ Sao Paulo, Inst Biociencias, R Matao 321, BR-05508090 Sao Paulo, SP - Brazil
[5] Univ N Carolina, Chapel Hill, NC 27599 - USA
Total Affiliations: 5
Document type: Journal article
Source: NEW PHYTOLOGIST; v. 231, n. 1, p. 365-381, JUL 2021.
Web of Science Citations: 1
Abstract

Moniliophthora perniciosa causes witches' broom disease of cacao and inflicts symptoms suggestive of hormonal imbalance. We investigated whether infection of the tomato (Solanum lycopersicum) model system Micro-Tom (MT) by the Solanaceae (S)-biotype of Moniliophthora perniciosa, which causes stem swelling and hypertrophic growth of axillary shoots, results from changes in host cytokinin metabolism. Inoculation of an MT-transgenic line that overexpresses the Arabidopsis CYTOKININ OXIDASE-2 gene (35S::AtCKX2) resulted in a reduction in disease incidence and stem diameter. RNA-sequencing analysis of infected MT and 35S::AtCKX2 revealed the activation of cytokinin-responsive marker genes when symptoms were conspicuous. The expression of an Moniliophthora perniciosa tRNA-ISOPENTENYL-TRANSFERASE suggests the production of isopentenyladenine (iP), detected in mycelia grown in vitro. Inoculated MT stems showed higher levels of dihydrozeatin and trans-zeatin but not iP. The application of benzyladenine induced symptoms similar to infection, whereas applying the cytokinin receptor inhibitors LGR-991 and PI55 decreased symptoms. Moniliophthora perniciosa produces iP that might contribute to cytokinin synthesis by the host, which results in vascular and cortex enlargement, axillary shoot outgrowth, reduction in root biomass and an increase in fruit locule number. This strategy may be associated with the manipulation of sink establishment to favour infection by the fungus. (AU)

FAPESP's process: 16/10524-5 - Transcriptional profile characterization in the Moniliophthora perniciosa x Solanum lycopersicum interaction
Grantee:Juliana Leles Costa
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/04309-6 - Functional analysis of cacao signalling and defense genes in the interaction Moniliophthora perniciosa x Solanum lycopersicum
Grantee:Juliana Leles Costa
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 19/12188-0 - Evaluation of the orchestrated pathways of miR156 in the interaction between Moniliophthora perniciosa and Solanum lycopersicum: consequences for pathogenesis and susceptibility
Grantee:Rafael Monteiro Do Carmo
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 16/10498-4 - Investigation of strategies of adaptation to the pathogenic life style of fungi from the Moniliophthora genus at various levels of biological organizations: species, biotypes, and geographic lineages
Grantee:Antonio Vargas de Oliveira Figueira
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/00060-9 - Evaluation of the role of plant hormones involved in differential response of tomato cv. Micro-Tom to fungi of genus Alternaria and Oidium through the change of transcripts
Grantee:Jamille Santos da Silva
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/17000-4 - Analysis of the interaction Moniliophthora perniciosa x 'Micro-Tom' tomato: non-host type resistance, effects on the development and role of phytohormones in pathogenesis
Grantee:Eder Marques da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral