Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon

Author(s):Show less -

Ribeiro, Geovani de Oliveira ^[1] ; Gill, Danielle Elise ^{[2, 3]} ; Athaide Ribeiro, Edcelha Soares D. ^[4] ; Costa Monteiro, Fred Julio ^[4] ; Morais, Vanessa S. ^{[2, 3]} ; Marcatti, Roberta ^{[2, 3]} ; Rego, Marlisson Octavio da S. ^[4] ; Araujo, Emerson Luiz Lima ^[5] ; Witkin, Steven S. ^[6] ; Villanova, Fabiola ^[1] ; Deng, Xutao ^{[7, 8]} ; Sabino, Ester Cerdeira ^{[2, 3]} ; Delwart, Eric ^{[7, 8]} ; Leal, Elcio ^[1] ; da Costa, Antonio Charlys ^{[2, 3]}

Affiliation

^[1] Univ Fed Para, Inst Ciencias Biol, Lab Diversidade Viral, BR-66075000 Belem, Para - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Molestias Infecciosas & Parasitarias, BR-05403000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Inst Med Trop, BR-05403000 Sao Paulo - Brazil

^[4] Hlth Surveillance Superintendence Amapa, Publ Hlth Lab Amapa LACEN AP, BR-68905230 Macapa - Brazil

^[5] Minist Hlth CGLAB DAEVS SVS MS Brasilia, Gen Coordinat Publ Hlth Labs Strateg Articulat, Dept Hlth Surveillance, BR-70719040 Brasilia, DF - Brazil

^[6] Weill Cornell Med, Dept Obstet & Gynecol, New York, NY 10065 - USA

^[7] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 - USA

^[8] Vitalant Res Inst, 270 Masonic Ave, San Francisco, CA 94118 - USA

• Document type: Journal article
• Source: Viruses-Basel; v. 13, n. 4 APR 2021.
• Web of Science Citations: 0

Abstract

Dengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology is a growing and increasingly invaluable tool for understanding the dispersal, persistence, and diversity of this impactful virus. In this study, plasma samples (n = 824) from individuals with symptoms consistent with an arboviral febrile illness were analyzed to identity the molecular epidemiological dynamics of DENV circulating in the Brazilian state of Amapa. Twelve DENV type 1 (DENV-1) genomes were identified, which were phylogenetically related to the BR4 lineage of genotype V. Phylodynamics analysis suggested that DENV-1 BR-4 was introduced into Amapa around early 2010, possibly from other states in northern Brazil. We also found unique amino acids substitutions in the DENV-1 envelope and NS5 protein sequences in the Amapa isolates. Characterization of the DENV-1 BR-4 sequences highlights the potential of this new lineage to drive outbreaks of dengue in the Amazon region. (AU)

• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/21706-5 - Viral metagenomics of mosquitoes captured in the State of São Paulo
• Grantee: Vanessa dos Santos Morais
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)