Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Role of Satellite DNAs in Genome Architecture and Sex Chromosome Evolution in Crambidae Moths

Full text
Author(s):
Cabral-de-Mello, Diogo C. [1, 2] ; Zrzava, Magda [1, 3] ; Kubickova, Svatava [4] ; Rendon, Pedro [5] ; Marec, Frantisek [1]
Total Authors: 5
Affiliation:
[1] Czech Acad Sci, Inst Entomol, Biol Ctr, Ceske Budejovice - Czech Republic
[2] UNESP Univ Estadual Paulista, Inst Biociencias IB, Dept Biol Geral & Aplicada, Rio Claro - Brazil
[3] Univ South Bohemia, Fac Sci, Ceske Budejovice - Czech Republic
[4] Vet Res Inst, Brno - Czech Republic
[5] IAEA TCLA Consultant USDA APHIS Moscamed Program, Guatemala City - Guatemala
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN GENETICS; v. 12, MAR 30 2021.
Web of Science Citations: 3
Abstract

Tandem repeats are important parts of eukaryotic genomes being crucial e.g., for centromere and telomere function and chromatin modulation. In Lepidoptera, knowledge of tandem repeats is very limited despite the growing number of sequenced genomes. Here we introduce seven new satellite DNAs (satDNAs), which more than doubles the number of currently known lepidopteran satDNAs. The satDNAs were identified in genomes of three species of Crambidae moths, namely Ostrinia nubilalis, Cydalima perspectalis, and Diatraea postlineella, using graph-based computational pipeline RepeatExplorer. These repeats varied in their abundance and showed high variability within and between species, although some degree of conservation was noted. The satDNAs showed a scattered distribution, often on both autosomes and sex chromosomes, with the exception of both satellites in D. postlineella, in which the satDNAs were located at a single autosomal locus. Three satDNAs were abundant on the W chromosomes of O. nubilalis and C. perspectalis, thus contributing to their differentiation from the Z chromosomes. To provide background for the in situ localization of the satDNAs, we performed a detailed cytogenetic analysis of the karyotypes of all three species. This comparative analysis revealed differences in chromosome number, number and location of rDNA clusters, and molecular differentiation of sex chromosomes. (AU)

FAPESP's process: 19/19069-7 - Investigating the evolution of satellite DNAs in holocentric chromosomes of pest species
Grantee:Diogo Cavalcanti Cabral de Mello
Support Opportunities: Regular Research Grants