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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Environmental Clonal Spread of Azole-Resistant Candida parapsilosis with Erg11-Y132F Mutation Causing a Large Candidemia Outbreak in a Brazilian Cancer Referral Center

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Author(s):
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Thomaz, Danilo Y. [1] ; de Almeida, Joao N. [2, 1, 3] ; Sejas, Odeli N. E. [4] ; Del Negro, Gilda M. B. [1] ; Carvalho, Gabrielle O. M. H. [1] ; Gimenes, Viviane M. F. [1] ; de Souza, Maria Emilia B. [4] ; Arastehfar, Amir [2] ; Camargo, Carlos H. [5] ; Motta, Adriana L. [3] ; Rossi, Flavia [3] ; Perlin, David S. [2] ; Freire, Maristela P. [4] ; Abdala, Edson [4] ; Benard, Gil [1]
Total Authors: 15
Affiliation:
[1] Univ Sao Paulo, Fac Med, Lab Med Mycol LIM 53, Inst Med Trop, Hosp Clin, BR-05403000 Sao Paulo - Brazil
[2] Hackensack Meridian Hlth, Ctr Discovery & Innovat, Nutley, NJ 07110 - USA
[3] Univ Sao Paulo, Fac Med, Hosp Clin, Cent Lab Div LIM 03, BR-05403010 Sao Paulo - Brazil
[4] Univ Sao Paulo, Canc Inst Sao Paulo State, Hosp Clin, Fac Med, BR-01246000 Sao Paulo - Brazil
[5] Adolfo Lutz Inst, Bacteriol Ctr, BR-01246000 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF FUNGI; v. 7, n. 4 APR 2021.
Web of Science Citations: 1
Abstract

Clonal outbreaks due to azole-resistant Candida parapsilosis (ARCP) isolates have been reported in numerous studies, but the environmental niche of such isolates has yet to be defined. Herein, we aimed to identify the environmental niche of ARCP isolates causing unremitting clonal outbreaks in an adult ICU from a Brazilian cancer referral center. C. parapsilosis sensu stricto isolates recovered from blood cultures, pericatheter skins, healthcare workers (HCW), and nosocomial surfaces were genotyped by multilocus microsatellite typing (MLMT). Antifungal susceptibility testing was performed by the EUCAST (European Committee for Antimicrobial Susceptibility Testing) broth microdilution reference method and ERG11 was sequenced to determine the azole resistance mechanism. Approximately 68% of isolates were fluconazole-resistant (76/112), including pericatheter skins (3/3, 100%), blood cultures (63/70, 90%), nosocomial surfaces (6/11, 54.5%), and HCW's hands (4/28, 14.2%). MLMT revealed five clusters: the major cluster contained 88.2% of ARCP isolates (67/76) collected from blood (57/70), bed (2/2), pericatheter skin (2/3), from carts (3/7), and HCW's hands (3/27). ARCP isolates were associated with a higher 30 day crude mortality rate (63.8%) than non-ARCP ones (20%, p = 0.008), and resisted two environmental decontamination attempts using quaternary ammonium. This study for the first time identified ARCP isolates harboring the Erg11-Y132F mutation from nosocomial surfaces and HCW's hands, which were genetically identical to ARCP blood isolates. Therefore, it is likely that persisting clonal outbreak due to ARCP isolates was fueled by environmental sources. The resistance of Y132F ARCP isolates to disinfectants, and their potential association with a high mortality rate, warrant vigilant source control using effective environmental decontamination. (AU)

FAPESP's process: 19/17851-0 - Genotyping by microsatellite analysis of Candida parapsilosis (sensu stricto) isolates obtained from cases of Invasive Candidiasis
Grantee:Gabrielle Oliveira Marques Haddad Carvalho
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/00231-3 - Early diagnosis of candidemia in critical ill patients: comparative analysis of real-time PCR, MALDI-TOF and detection of 1,3-beta-D-glucan assay
Grantee:Gilda Maria Barbaro Del Negro
Support Opportunities: Regular Research Grants
FAPESP's process: 18/19347-4 - Characterization of molecular mechanisms related to azoles resistance in Trichosporon asahii clinical isolates
Grantee:João Nóbrega de Almeida Júnior
Support Opportunities: Scholarships abroad - Research
FAPESP's process: 16/08730-6 - Fungal pathogenicity: environmental effects, immune response and vaccine modulation in the Brazilian endemic mycoses paracoccidioidomycosis and histoplasmosis
Grantee:Carlos Pelleschi Taborda
Support Opportunities: Research Projects - Thematic Grants