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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bone demineralization improves onlay graft consolidation: A histological study in rat calvaria

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Carvalho, Erika B. S. [1] ; Veronesi, Giovana F. [1] ; Manfredi, Gustavo G. P. [1] ; Damante, Carla A. [1] ; Sant'Ana, Adriana C. P. [1] ; Greghi, Sebastiao L. A. [1] ; Zangrando, Mariana S. R. [1] ; Consolaro, Alberto [2] ; Rezende, Maria L. R. [1]
Total Authors: 9
[1] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodont, Bauru, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Div Pathol, Dept Stomatol, Bauru, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Periodontology; v. 92, n. 6, p. 1-10, JUN 2021.
Web of Science Citations: 0

Background Previous data suggest that bone demineralization may promote bone graft consolidation as well as proliferation and differentiation of pre-osteoblasts, but the biological mechanisms involved in this process need to be clarified. This study investigated the effects of bone demineralization with citric acid (CA) and tetracycline (TCN) on the repair of onlay bone grafts. Methods Onlay bone grafts were performed on the calvaria of 126 Wistar rats. The contacting surfaces between bone graft and receptor bone bed were demineralized for 15, 30, and 60 seconds with TCN (50 mg/mL), or 10% CA, (pH 1), constituting the following test groups (n = 18): TCN15, TCN30, TCN60, CA15, CA30, and CA60. Control grafts (C) were performed without demineralization (n = 18). After 7, 30, and 60 days, biopsies were obtained for quantitative and qualitative histological analysis (a = 6). Results Demineralization accelerated the bone repair early from 7 days of healing. Higher percentage area of newly formed bone was observed in CA15 and TCN60 groups when compared to C in all evaluation periods (P = 0.02). At 30 days, C specimens had lower percentage of consolidated surfaces than TCN60, TCN30 and CA15 (P = 0.0015). At 60 days, CA15, CA60, and TCN60 presented bone surfaces almost completely filled by newly formed bone, against about 75% in C specimens (P = 0.0015). Conclusions Both CA and TCN were effective in accelerating osteogenesis at the interface between bone grafts and receptor bone beds, especially when applied for 15 seconds and 60 seconds, respectively. (AU)

FAPESP's process: 14/15136-8 - Effect of bone demineralization on tissue repair processes: microscopic, structural and osteoblastic cells activity study in rats calvariae
Grantee:Maria Lúcia Rubo de Rezende
Support type: Regular Research Grants