Identification of bioactive peptides released from in vitro gastrointestinal digestion of yam proteins (Dioscorea cayennensis)

Author(s):Show less -

do Nascimento, Edilza Silva ^{[1, 2]} ; Anaya, Katya ^[3] ; de Oliveira, Julia Mariano Caju ^[2] ; de Lacerda, Jose Thalles Jocelino Gomes ^[4] ; Miller, Michael Edward ^[5] ; Dias, Meriellen ^[6] ; Mendes, Maria Anita ^[6] ; Pallone, Juliana de Azevedo Lima ^[7] ; Arns, Clarice Weis ^[5] ; Juliano, Maria Aparecida ^[4] ; Gadelha, Tatiane Santi ^[8] ; Pacheco, Maria Teresa Bertoldo ^[9] ; Gadelha, Carlos Alberto de Almeida ^{[1, 2]}

Affiliation

^[1] Univ Fed Paraiba, Technol Ctr, Postgrad Program Food Sci & Technol, Dept Food Engn, Joao Pessoa, PB - Brazil

^[2] Univ Fed Paraiba, Lab Struct Prote, Dept Mol Biol, BR-58051900 Joao Pessoa, PB - Brazil

^[3] Univ Fed Rio Grande do Norte, Fac Hlth Sci Trari, Santa Cruz, RN - Brazil

^[4] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo, SP - Brazil

^[5] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut & Bioagents, Campinas, SP - Brazil

^[6] Univ Sao Paulo, Dept Chem Engn, Sao Paulo, SP - Brazil

^[7] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, Campinas, SP - Brazil

^[8] Univ Fed Paraiba, Lab Genet Biochem & Radiol, Dept Mol Biol, Joao Pessoa, PB - Brazil

^[9] Ctr Food Sci & Qual, Inst Food Technol, Campinas, SP - Brazil

• Document type: Journal article
• Source: Food Research International; v. 143, MAY 2021.
• Web of Science Citations: 0

Abstract

Bioactive peptides have been broadly studied for their contribution to human health. This study aimed to identify bioactive peptides generated by in vitro gastrointestinal digestion of yam proteins. Yam protein concentrate (YPC) was submitted to simulated digestion. Gastric phase hydrolysate (GPH) and total gastrointestinal phase hydrolysate (GIPH) had their peptides identified by nanoLC-ESI-MS/MS. Peptide sequences were subjected to a database-driven (BIOPEP) bioactivity search. In vitro tests included: Antioxidant activity, DNA damage protection, ACE-inhibitory activity and antibacterial activity against the bacteria Escherichia coli, Salmonella sp. and Lysteria monocytogenes. Simulated digestion generated small peptides (mostly MW < 3500 Da), several of them with potential bioactive sequences predicted in silico. In both GPH and GIPH biological activities were detected, although GIPH displayed stronger DNA damage protection and antibacterial activity against Escherichia coli. The digestion of yam proteins releases promising biologically active peptides which can contribute to the prevention of bacterial infection and chronic degenerative diseases, with beneficial effects to human health. (AU)

• FAPESP's process: 12/50191-4 - Synthesis, kinetic studies and applications of substrates and inhibitors for proteolytic enzymes
• Grantee: Maria Aparecida Juliano
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/13588-0 - Multi - user equipment approved in grant 2012/50191-4: mass spectrometer impact II - Bruker
• Grantee: Maria Aparecida Juliano
• Support Opportunities: Multi-user Equipment Program