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Partial hydrolysates of whey proteins from bovine and buffalo milk obtained with immobilised proteases: peptides transportation and absorption through dialyzability and Caco-2 cell culture

Grant number: 12/07680-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2013
Effective date (End): July 31, 2015
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Rubens Monti
Grantee:Juliana Cristina Bassan
Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


This project aims to obtain peptides from bovine and buffalo whey proteins by enzymatic hydrolysis and apply them to in vitro gastrointestinal digestion simulation for dialyzability and culture of Caco-2 cells assays. The hydrolysates are obtained by using commercial proteases trypsin and pepsin immobilized on agarose, and corn cob powder and/or coconut fiber as alternatives low cost supports. Enzymatic hydrolysis with active and stabilized derivatives (enzyme linked to the support) under pH and temperature conditions predetermined will be conducted in fluidized bed reactor to make possible to easily separate the product from the reaction medium; the recycling and reuse of the derivatives will be also studied. For hydrolysis, the derivatives will be used in different combinations, allowing the release of different products. These will be later analyzed by chromatographic methods aiming the isolation of peptides. Once isolated, the peptides will undergo gastrointestinal conditions in order to verify its integrity. These studies could provide future projects to study possible biological and bioactive properties of these fragments and their applications in various food and pharmaceutical industries.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA CRUZ, CLARIANA ZANUTTO PAULINO; DE MENDONCA, RICARDO JOSE; GUIMARAES, LUIS HENRIQUE SOUZA; DOS SANTOS RAMOS, MATHEUS APARECIDO; GARRIDO, SAULO SANTESSO; DE PAULA, ARIELA VELOSO; MONTI, RUBENS; MASSOLINI, GABRIELLA. Assessment of the Bioactive Potential of Cheese Whey Protein Hydrolysates Using Immobilized Alcalase. Food and Bioprocess Technology, v. 13, n. 12, p. 2120-2130, . (12/07680-4, 14/12563-2)
BASSAN, JULIANA CRISTINA; DE SOUZA BEZERRA, THAIS MILENA; PEIXOTO, GUILHERME; PAULINO DA CRUZ, CLARIANA ZANUTTO; MARTINEZ GALAN, JULIAN PAUL; DOS SANTOS VAZ, ALINE BUDA; GARRIDO, SAULO SANTESSO; FILICE, MARCO; MONTI, RUBENS. Immobilization of Trypsin in Lignocellulosic Waste Material to Produce Peptides with Bioactive Potential from Whey Protein. MATERIALS, v. 9, n. 5, . (12/07680-4, 14/12563-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BASSAN, Juliana Cristina. Hidrolisados parciais de soro proteínas lácteas bovina e bubalina obtidos com proteases imobilizadas: ensaios de transporte e absorção de peptídeos através das técnicas da dialisabilidade e cultura de células caco-2. 2015. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.

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