Milk Macrophage Function in Bovine Leukemia Virus-Infected Dairy Cows

Author(s):Show less -

Lima, Ewerton de Souza ^{[1, 2]} ; Blagitz, Maiara Garcia ^{[3, 4]} ; Batista, Camila Freitas ^[3] ; Alves, Alexandre Jose ^[1] ; Fernandes, Artur Cezar de Carvalho ^{[1, 2]} ; Ramos Sanchez, Eduardo Milton ^{[5, 6]} ; Frias Torres, Hugo ^[7] ; Diniz, Soraia Araujo ^[8] ; Silva, Marcos Xavier ^[8] ; Della Libera, Alice Maria Melville Paiva ^[3] ; de Souza, Fernando Nogueira ^{[1, 2, 3]}

Affiliation

^[1] Univ Fed Paraiba, Ctr Ciencias Agr, Dept Med Vet, Nucleo Aplicado Prod & Sanidade Glandula Mamaria, Areia - Brazil

^[2] Univ Fed Paraiba, Ctr Ciencias Agr, Programa Posgrad Ciencia Anim, Areia - Brazil

^[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, Sao Paulo - Brazil

^[4] Univ Fed Fronteira Sul, Programa Posgrad Saude Bemestar & Prod Anim Suste, Ave Edmundo Gaievski 1000, Realeza - Brazil

^[5] Natl Univ Toribio Rodriguez Mendonza Amazonas, Sch Hlth Sci, Dept Publ Hlth, Chachapoyas - Peru

^[6] Univ Sao Paulo, Inst Med Trop, Lab Sorol & Imunobiol, Sao Paulo - Brazil

^[7] Univ Nacl Toribio Rodriguez Mendoza, Anim Husb & Biotechnol Res Inst, Parasit & Infect Dis Lab, Chachapoyas - Peru

^[8] Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, Belo Horizonte, MG - Brazil

• Document type: Journal article
• Source: FRONTIERS IN VETERINARY SCIENCE; v. 8, JUN 17 2021.
• Web of Science Citations: 1

Abstract

The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production of reactive oxygen and nitrogen species (RONS) by macrophages in milk samples from dairy cows naturally infected with BLV with or without persistent lymphocytosis (PL). No effect of BLV infection in the overall number of macrophages per milliliter and in the percentage of viable macrophages among overall milk viable cells was found. Furthermore, BLV-infected dairy cows had a higher frequency of viable milk macrophages, while healthy animals had a tendency toward a higher percentage of apoptotic milk macrophages. The percentage of milk macrophages that phagocytosed Staphylococcus aureus in seronegative animals was higher than that in BLV-infected dairy cows. No effect of BLV infection on the intracellular RONS production and the intensity of phagocytosis by milk macrophages was observed. Thus, this study provides new insights into the implications of BLV infections in the bovine mammary gland. (AU)

• FAPESP's process: 09/50672-0 - Functional evaluation of blood and milk leukocytes of cows naturally infected by bovine leukosis virus
• Grantee: Alice Maria Melville Paiva Della Libera
• Support Opportunities: Regular Research Grants