Nanoformulation Shows Cytotoxicity against Glioblastoma Cell Lines and Antiangiogenic Activity in Chicken Chorioallantoic Membrane

Author(s):Show less -

Dallemole, Danieli Rosane ^[1] ; Terroso, Thatiana ^[1] ; Alves, Aline de Cristo Soares ^[1] ; Scholl, Juliete Nathali ^[2] ; Onzi, Giovana Ravizzoni ^[1] ; Ce, Rodrigo ^[1] ; Paese, Karina ^{[1, 3]} ; Battastini, Ana Maria Oliveira ^[2] ; Guterres, Silvia Staniscuaski ^{[1, 3]} ; Figueiro, Fabricio ^[4] ; Pohlmann, Adriana Raffin ^[1]

Affiliation

^[1] Univ Fed Rio Grande do Sul, Fac Pharm, Grad Program Pharmaceut Sci, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil

^[2] Univ Fed Rio Grande do Sul, Inst Hlth Sci, Grad Program Biol Sci Biochem, Ramiro Barcelos St 2600, BR-90035003 Porto Alegre, RS - Brazil

^[3] Univ Fed Rio Grande do Sul, Dept Prod & Control Med, Av Ipiranga 2752, BR-90610000 Porto Alegre, RS - Brazil

^[4] Univ Fed Rio Grande do Sul, Inst Hlth Sci, Dept Biochem, Ramiro Barcelos St 2600, BR-90035003 Porto Alegre, RS - Brazil

• Document type: Journal article
• Source: PHARMACEUTICS; v. 13, n. 6 JUN 2021.
• Web of Science Citations: 0

Abstract

Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that is highly proliferative and vascularized. The prognosis is poor with currently available treatment. In this study, we evaluated the cytotoxicity and antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(epsilon-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). The nanoformulation was prepared by self-assembling followed by interfacial reactions, physicochemically characterized and evaluated in vitro against GB cell lines (U87MG and U138MG) and in vivo using the chicken chorioallantoic membrane assay (CAM). Spherical shape nanocapsules had a hydrodynamic mean diameter of 138 nm, zeta potential of +13.4 mV, doxorubicin encapsulation of 65%, and RGD conjugation of 92%. After 24 h of treatment (U87MG and U138MG), the median inhibition concentrations (IC50) were 520 and 490 nmol L-1 doxorubicin-equivalent concentrations, respectively. The treatment induced antiproliferative activity with S-phase cell-cycle arrest and apoptosis in the GB cells. Furthermore, after 48 h of exposure, evaluation of antiangiogenic activity (CAM) showed that the relative vessel growth following treatment with the nanocapsules was 5.4 times lower than that with the control treatment. The results support the therapeutic potential of the nanoformulation against GB and, thereby, pave the way for future preclinical studies. (AU)

• FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
• Grantee: Maria Vitória Lopes Badra Bentley
• Support Opportunities: Research Projects - Thematic Grants