Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure

Author(s)

Gomes, Brenda P. F. A. ^[1] ; Francisco, Priscila Amanda ^[1] ; Godoi Jr, Ederaldo Pietrafesa ; Endo, Marcos Sergio ^{[2, 3]} ; Barbosa-Ribeiro, Marlos ^{[2, 4]} ; Delboni, Maraisa Greggio ^{[2, 5]} ; Pecorari, Vanessa G. A. ^{[6, 2]}

Affiliation

^[1] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Div Endodont, Piracicaba, SP - Brazil

^[2] Godoi Jr, Jr., Ederaldo Pietrafesa, Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Restorat Dent, Div Endodont, Piracicaba, SP - Brazil

^[3] Univ Estadual Maringa, UEL, Hlth Sci Ctr, Dept Dent, Maringa, PR - Brazil

^[4] Univ Pernambuco, Sch Dent, Arcoverde Campus, Arcoverde, PE - Brazil

^[5] DeVry Facid Univ, Coll Dent, Teresina, PI - Brazil

^[6] Univ Paulista, Sch Dent, Dent Res Div, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 47, n. 7, p. 1075-1086, JUL 2021.
• Web of Science Citations: 1

Abstract

Aim: To elucidate the presence of apical periodontitis in the root canal of teeth with secondary/persistent infection, including composition of microbiota, levels of endotoxins and lipoteichoic acid (LTA), and clinical implications of these findings. Method: Samples were collected from root canals of 50 patients who needed endodontic retreatment and had radiographic evidence of apical periodontitis. Microorganisms were identified by using the culture technique and biochemical tests. Nested-polymerase chain reaction (nested-PCR) was used to identify 17 species of specific bacteria. Lipopolysaccharides (LPS) and LTAs were quantified by using, respectively, limulus amebocyte lysate and enzyme-linked immunosorbent assay tests. Results: Bacteria were detected in all samples by culture and molecular methods. A total of 154 gram-positive strains, of 188 strains isolated, were found in the root canals by culture. Enterococcus faecalis and Gemella morbilorum were the most prevalent species identified by the biochemical tests, whereas molecular analyses (nestedPCR) showed a high frequency of P. gingivalis, E. faecalis, and Fusobacterium nucleatum. LPS and LTA were detected in all samples, with mean values being 3.52 EU/mL and 597.83 pg/mL, respectively. Significant statistical correlations were found between levels of LTA and clinical features. Conclusion: Despite the prevalence of gram-positives, the microbiota present in secondary/persistent infections showed a large variety of species. Within this diversity, associations were found between specific bacteria and clinical features. In addition, higher levels of LTA were statistically associated with larger periapical radiolucent areas, but no correlation between this feature and LPS was found. (J Endod 2021;47:1075-1086.) (AU)

• FAPESP's process: 15/19215-2 - Microorganisms and their virulence factors detection in saliva, pulp chamber and root canal of teeth associated with endodontic failure
• Grantee: Priscila Amanda Francisco
• Support Opportunities: Scholarships in Brazil - Master