Development of magnetic nanoparticles modified with new molecularly imprinted polymer (MIPs) for selective analysis of glutathione

Author(s)

Santos, Ana Caroline Ferreira ^[1] ; de Araujo, Orlando R. P. ^[1] ; Moura, Fabiana A. ^[1] ; Khan, Sabir ^{[2, 3]} ; Tanaka, Auro A. ^[4] ; Santana, Antonio Euzebio G. ^[5] ; Pividori, Maria Isabel ^[6] ; Taboada-Sotomayor, Maria del Pilar ^{[2, 3]} ; Goulart, Marilia O. F. ^[1]

Affiliation

^[1] Univ Fed Alagoas, Inst Quim Biotecnol, Campus AC Simoes, BR-57072970 Maceio, Alagoas - Brazil

^[2] Univ Estadual Paulista, Inst Quim, Rua Prof Francisco Degni 55, Araraquara, SP - Brazil

^[3] INCT DATREM, Rua Prof Francisco Degni 55, Araraquara, SP - Brazil

^[4] Univ Fed Maranhao, Dept Quim, Ave Portugueses 1966, BR-65080805 Sao Luis, Maranhao - Brazil

^[5] Univ Fed Alagoas, Campus Engn Ciencias Agr, BR-57072970 Maceio, Alagoas - Brazil

^[6] Univ Autonoma Barcelona, Bellaterra 08193, Barcelona - Spain

• Document type: Journal article
• Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 344, OCT 1 2021.
• Web of Science Citations: 0

Abstract

The present work aims to develop electroanalytical strategies for separating and quantifying one of the most important biothiols, the reduced glutathione (GSH), based on developing and applying a new and selective magnetic molecular imprinted polymer (mag-MIP). This magnetic-supported polymer was synthesized by precipitation, using GSH as a template, having acrylamide as the functional monomer and trimethylolpropane trimethacrylate as a cross-linking agent. These materials' morphological and physical characteristics were investigated by scanning electron microscope, Fourier transform infrared, and vibrating sample magnetometry. Physicochemical parameters, such as adsorption capacity, were obtained and compared with the respective magNIP, which are control polymers without the selective cavity. After all characterizations, mag-MIP and mag-NIP were evaluated on magnetic graphite-epoxy composite (m-GEC) electrodes. The characteristics of the obtained sensors were investigated by cyclic voltammetry and differential pulse voltammetry. After optimizing experimental and operational conditions, a linear response for the concentration of GSH, in the range of 1-1400 mu mol/ L, with low detection and quantification limits of 7 and 20 nmol/L, respectively, were obtained. The GSH levels in mice liver samples were measured by electrochemical and spectrophotometric techniques to compare the method's effectiveness. A high correlation between them and a low relative error of 1.06 % were obtained, indicating the good functionality of the proposed sensor. (AU)

• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants