Influence of Bacterial Profiles in Cytokine and Clinical Features of Endodontic Disease

Author(s)

Martinho, Frederico C. ^{[1, 2]} ; Leite, Fabio R. M. ^[3] ; Arruda-Vasconcelos, Rodrigo ^[1] ; Darveau, Richard P. ^{[1, 4]} ; Gomes, Brenda P. F. A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Div Endodont, Dept Restorat Dent, Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Maryland, Sch Dent, Div Endodont, Dept Adv Oral Sci & Therapeut, 650 West Baltimore St, 6th Floor, Suite 6253, Baltimore, MD 21201 - USA

^[3] Aarhus Univ, Sect Periodontol, Dept Dent & Oral Hlth, Aarhus - Denmark

^[4] Univ Washington, Dept Periodont, Seattle, WA - USA

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 47, n. 8, p. 1265-1271, AUG 2021.
• Web of Science Citations: 0

Abstract

Introduction: We verified the association between selected bacterial profiles and levels of cytokines, chemokines, and the expression of signs and symptoms of primary endodontic infection with apical periodontitis. Methods: Samples were collected from 21 root canals, and macrophages were stimulated for 24 hours. Tumor necrosis factor alpha (TNF-a), interleukin (IL)-6, IL-10, IL-12p70, interferon gamma, and chemokine (C-C motif) ligand 2 (CCL2) were measured using cytometric bead array. We investigated the overlapping net-works between cytokines and chemokines with regression analysis. Checkerboard DNA -DNA hybridization was used to assess 40 target bacteria species. Using factor analysis, bacterial species aggregated in 2 factors. The association of bacteria species-based factors on cytokine and chemokine levels and clinical features was estimated with regression analysis. Results: A negative relationship between IL-10 (anti-inflammatory cytokine) and CCL2, TNF-a, and IFN-g (proinflammatory cytokines) (all P < .05) was observed. CCL2 was positively correlated with TNF-a (P < .01). Thirty-eight bacteria species were detected in primary endodontic infection with apical periodontitis. The first bacteria species-based factor was associated with the size of the radiolucent area (coefficient = 15.42) and tenderness to percussion/pain on palpation (coefficient = 20.79). The second factor was associated with CCL2 levels (coefficient = 1.28). Conclusions: Different bacterial profiles can be differentially related to the expression of inflammatory proteins and the experience of clinical features. (AU)

• FAPESP's process: 19/10755-5 - Clinical investigation of epigenetic modulations in dental pulp tissues with diagnosis of irreversible pulpitis
• Grantee: Rodrigo Arruda Vasconcelos
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/23479-5 - Microbiomes and immunobiological aspects in endodontic infections
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/25242-8 - Clinical, microbiological and immunological monitoring of patients undertaken to endodontic therapy with diagnosis of irreversible Pulpitis
• Grantee: Rodrigo Arruda Vasconcelos
• Support Opportunities: Scholarships in Brazil - Doctorate