Microbiome and oral squamous cell carcinoma: a possible interplay on iron metabolism and its impact on tumor microenvironment

Author(s):Show less -

Arthur, Rodrigo Alex ^[1] ; Bezerra, Rafael dos Santos ^{[2, 3]} ; Bianchi Ximenez, Joao Paulo ^{[3, 4]} ; Merlin, Bruna Lais ^[5] ; Morraye, Raphael de Andrade ^{[6, 3, 4]} ; Neto, Joao Valentini ^[7] ; Nasser Fava, Natalia Melo ^[8] ; Alves Figueiredo, David Livingstone ^{[9, 10]} ; Oliveira de Biagi Jr, Carlos Alberto ; Montibeller, Maria Jara ^[11] ; Guimaraes, Jhefferson Barbosa ^[12] ; Alves, Ellen Gomes ^[13] ; Schreiner, Monique ^[14] ; da Costa, Tiago Silva ^[15] ; Liberati da Silva, Charlie Felipe ^[14] ; Malheiros, Jessica Moraes ^[16] ; Burda da Silva, Luan Henrique ^[14] ; Ribas, Guilherme Taborda ^[14] ; Achallma, Daisy Obispo ^{[17, 18]} ; Braga, Camila Margalho ^[19] ; Assis Andrade, Karen Flaviane ^[20] ; Alves Martins, Valquiria do Carmo ^[21] ; Nestor dos Santos, Glauco Vinicius ^[22] ; Granatto, Caroline Fabiane ^[9] ; Terin, Ulisses Costa ^[9] ; Sanches, Igor Henrique ^[22] ; Ramos, Diana Estefania ^[23] ; Garay-Malpartida, Humberto Miguel ^[24] ; Pereira de Souza, Gabriela Marcelino ^[25] ; Slavov, Svetoslav Nanev ^[25] ; Silva Jr, Wilson Araujo ; 2020, Summer Course

Affiliation:Show less -

^[1] Univ Fed Rio Grande do Sul, Fac Dent, Prevent & Community Dent Dept, BR-90035003 Porto Alegre, RS - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Postgrad Program Clin Oncol Stem Cells & Cell The, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Reg Blood Ctr Ribeirao Preto, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Based Therapy CEPID FAPESP, Mol Genet & Bioinfonnat Lab MGBL, Rua Tenente Catao Roxo 2501, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirio Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP - Brazil

^[6] Univ Sao Paulo, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP - Brazil

^[7] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo, SP - Brazil

^[8] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, BR-13563120 Sao Carlos, SP - Brazil

^[9] Inst Canc Res IPEC, BR-85015430 Guarapuava, PR - Brazil

^[10] Univ Estadual Centro Oeste, Dept Med, BR-85015430 Guarapuava, PR - Brazil

^[11] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Food & Nutr, Araraquara, SP - Brazil

^[12] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto, SP - Brazil

^[13] Univ Paulista, Inst Hlth Sci, Undergrad Biol Sci, Ribeirao Preto, SP - Brazil

^[14] Univ Fed Parana, Grad Program Bioinformat Profess & Technol Educ S, Curitiba, Parana - Brazil

^[15] Univ Fed Amapa, Dept Biol Sci & Hlth, Macapa, AP - Brazil

^[16] Embrapa Southeast Livestock EMBRAPA, Sao Paulo - Brazil

^[17] FARVET, Labs Invest & Desarrollo, Chincha Alta, Ica - Peru

^[18] Univ San Martin de Porres, Ctr Invest Genet & Biol Mol CIGBM, Lima - Peru

^[19] Para State Univ, Grad Program Parasit Biol Amazon, Belem, Para - Brazil

^[20] Para State Univ, Inst Technol, Dept Elect & Biomed Engn, Belem, Para - Brazil

^[21] Fundacao Ctr Controle Oncol Estado Amazonas, Dept Educ & Res, Manaus, Amazonas - Brazil

^[22] Univ Fed Goias, Inst Pathol Trop & Publ Hlth, Goiania, Go - Brazil

^[23] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral Maxillofacial Surg & Periodontol, Ribeirao Preto, SP - Brazil

^[24] Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo - Brazil

^[25] Oliveira de Biagi Jr, Jr., Carlos Alberto, Reg Blood Ctr Ribeirao Preto, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Based Therapy CEPID FAPESP, Mol Genet & Bioinfonnat Lab MGBL, Rua Tenente Catao Roxo 2501, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 52, n. 3, p. 1287-1302, SEP 2021.
• Web of Science Citations: 0

Abstract

There is increasing evidence showing positive association between changes in oral microbiome and the occurrence of oral squamous cell carcinoma (OSCC). Alcohol- and nicotine-related products can induce microbial changes but are still unknown if these changes are related to cancerous lesion sites. In an attempt to understand how these changes can influence the OSCC development and maintenance, the aim of this study was to investigate the oral microbiome linked with OSCC as well as to identify functional signatures and associate them with healthy or precancerous and cancerous sites. Our group used data of oral microbiomes available in public repositories. The analysis included data of oral microbiomes from electronic cigarette users, alcohol consumers, and precancerous and OSCC samples. An R-based pipeline was used for taxonomic and functional prediction analysis. The Streptococcus spp. genus was the main class identified in the healthy group. Haemophilus spp. predominated in precancerous lesions. OSCC samples revealed a higher relative abundance compared with the other groups, represented by an increased proportion of Fusobacterium spp., Prevotella spp., Haemophilus spp., and Campylobacter spp. Venn diagram analysis showed 52 genera exclusive of OSCC samples. Both precancerous and OSCC samples seemed to present a specific associated functional pattern. They were menaquinone-dependent protoporphyrinogen oxidase pattern enhanced in the former and both 3 `,5 `-cyclic-nucleotide phosphodiesterase (purine metabolism) and iron(III) transport system ATP-binding protein enhanced in the latter. We conclude that although precancerous and OSCC samples present some differences on microbial profile, both microbiomes act as ``iron chelators-like{''} potentially contributing to tumor growth. (AU)

• FAPESP's process: 19/08528-0 - Application of bioinformatic procedures for identification of the impact of the viral infections in hemotherapy
• Grantee: Rafael dos Santos Bezerra
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/23205-8 - Evaluation of the impact of emerging and reemerging viruses in the field of hemotherapy and stem cell transplantation by multiple-research approach
• Grantee: Svetoslav Nanev Slavov
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC