International clones of extended-spectrum beta-lactamase (CTX-M)-producing Escherichia coli in peri-urban wild animals, Brazil

Author(s)

de Carvalho, Marcelo P. N. ^[1] ; Fernandes, Miriam R. ^[2] ; Sellera, Fabio P. ^[3] ; Lopes, Ralf ^[4] ; Monte, Daniel F. ^[5] ; Hippolito, Alicia G. ^[6] ; Milanelo, Liliane ^[7] ; Raso, Tania F. ^[8] ; Lincopan, Nilton ^{[4, 2]}

Affiliation

^[1] Univ Fed Minas Gerais, Dept Vet Clin & Surg, Belo Horizonte, MG - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Pharmaceut Sci, Food Res Ctr, Dept Food & Expt Nutr, Sao Paulo - Brazil

^[6] Univ Estadual Paulista, Sch Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, UNESP, Botucatu, SP - Brazil

^[7] Recept Ctr Wildlife, Ecol Pk Tiete, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: TRANSBOUNDARY AND EMERGING DISEASES; v. 67, n. 5, p. 1804-1815, SEP 2020.
• Web of Science Citations: 4

Abstract

CTX-M-type extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli clones have been increasingly reported worldwide. In this regard, although discussions of transmission routes of these bacteria are in evidence, molecular data are lacking to elucidate the epidemiological impacts of ESBL producers in wild animals. In this study, we have screened 90 wild animals living in a surrounding area of Sao Paulo, the largest metropolitan city in South America, to monitor the presence of multidrug-resistant (MDR) Gram-negative bacteria. Using a genomic approach, we have analysed eight ceftriaxone-resistant E. coli. Resistome analyses revealed that all E. coli strains carried bla(CTX-M)-type genes, prevalent in human infections, besides other clinically relevant resistance genes to aminoglycosides, beta-lactams, phenicols, tetracyclines, sulphonamides, trimethoprim, fosfomycin and quinolones. Additionally, E. coli strains belonged to international sequence types (STs) ST38, ST58, ST212, ST744, ST1158 and ST1251, and carried several virulence-associated genes. Our findings suggest spread and adaptation of international clones of CTX-M-producing E. coli beyond urban settings, including wildlife from shared environments. (AU)

• FAPESP's process: 15/13527-2 - Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods
• Grantee: Miriam Rodriguez Fernandes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/03044-7 - Genetic diversity, virulence genes and antimicrobial resistance of Salmonella spp. isolated from poultry slaughterhouse
• Grantee: Daniel Farias Marinho Do Monte
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants