Texturized P(VDF-TrFE)/BT membrane enhances bone neoformation in calvaria defects regardless of the association with photobiomodulation therapy in ovariectomized rats

Author(s):Show less -

Toloi Rufato, Fernanda Cristina ^[1] ; de Sousa, Luiz Gustavo ^[1] ; Scalize, Priscilla Hakime ^[1] ; Gimenes, Rossano ^[2] ; Regalo, Isabela Hallak ^[1] ; Rosa, Adalberto Luiz ^[3] ; Beloti, Marcio Mateus ^[1] ; de Oliveira, Fabiola Singaretti ^[3] ; Bombonato-Prado, Karina Fittipaldi ^[1] ; Hallak Regalo, Simone Cecilio ^[1] ; Siessere, Selma ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Itajuba, Inst Phys & Chem, Itajuba, MG - Brazil

^[3] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 1 AUG 2021.
• Web of Science Citations: 0

Abstract

Objectives The purpose of this investigation was to evaluate in vivo the response of bone tissue to photobiomodulation when associated with texturized P(VDF-TrFE)/BT in calvaria defects of ovariectomized rats. Materials and methods Wistar Hannover rats were submitted to ovariectomy/control surgery. Calvaria bone defects of 5-mm diameter were performed after 90 days of ovariectomy. The animals were divided into OVX (without laser (L) and membrane), OVX + P(VDF-TrFE)/BT, OVX + P(VDF-TrFE)/BT + L, and OVX + PTFE + L. It was utilized a low-intensity gallium-aluminum-arsenide laser (GaAlAs) with 780-nm wavelength and 30-J/cm(2) energy density in 12 sessions (120 s). Thirty days after the bone defect the animals were euthanized for histological, microtomographic, and molecular evaluation. Quantitative analysis was analyzed by statistical software for p < 0.05. Results Histological parameters showed bone tissue formation at the borders of all group defects. The association of photobiomodulation and texturized P(VDF-TrFE)/BT was not synergistic and did not show significant changes in morphometric analysis and biomarkers gene expression. Nevertheless, texturized P(VDF-TrFE)/BT membrane enhanced bone repair regardless of the association with photobiomodulation therapy, with an increase of connectivity density when compared to the OVX + PTFE + L group. The association of photobiomodulation therapy and PTFE was synergistic, increasing the expression of Runx2, Alp, Bsp, Bglap, Sp7, and Rankl, even though not enough to reflect significance in the morphometric parameters. Conclusions The utilization of texturized P (VDF-TrFE)/BT, regardless of the association with photobiomodulation therapy, enhanced bone repair in an experimental model of osteoporosis. (AU)

• FAPESP's process: 17/25683-4 - The effect of association of low-level laser irradiation and poly(vinylidene-trifluoroethylene)/barium titanate composite in the repair of bone defects in calvaria of ovariectomized rats.
• Grantee: Selma Siéssere
• Support Opportunities: Regular Research Grants