| Full text | |
| Author(s): |
Fidelis, Carlos E.
[1]
;
Franke, Manoela
[1]
;
de Abreu, Leticia C. R.
[1]
;
Jagielski, Tomasz
[2]
;
Ribeiro, Marcio G.
[3]
;
dos Santos, V, Marcos
;
Goncalves, Juliano L.
[4, 5]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, Pirassununga, SP - Brazil
[2] Univ Warsaw, Fac Biol, Inst Microbiol, Dept Med Microbiol, Warsaw - Poland
[3] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Prod & Prevent Vet Med, UNESP, Botucatu, SP - Brazil
[4] Michigan State Univ, Coll Vet Med, Dept Large Anim Clin Sci, E Lansing, MI 48824 - USA
[5] dos Santos, Marcos, V, Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, Pirassununga, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION; v. 33, n. 6 AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of Sao Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases. (AU) | |
| FAPESP's process: | 17/50461-5 - Bovine mastitis: control by local use of polyplex nanoparticles |
| Grantee: | Marcos Veiga dos Santos |
| Support Opportunities: | Regular Research Grants |