MALDI-TOF MS identification of Prototheca algae associated with bovine mastitis

Author(s)

Fidelis, Carlos E. ^[1] ; Franke, Manoela ^[1] ; de Abreu, Leticia C. R. ^[1] ; Jagielski, Tomasz ^[2] ; Ribeiro, Marcio G. ^[3] ; dos Santos, V, Marcos ; Goncalves, Juliano L. ^{[4, 5]}

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, Pirassununga, SP - Brazil

^[2] Univ Warsaw, Fac Biol, Inst Microbiol, Dept Med Microbiol, Warsaw - Poland

^[3] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Prod & Prevent Vet Med, UNESP, Botucatu, SP - Brazil

^[4] Michigan State Univ, Coll Vet Med, Dept Large Anim Clin Sci, E Lansing, MI 48824 - USA

^[5] dos Santos, Marcos, V, Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, Pirassununga, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION; v. 33, n. 6 AUG 2021.
• Web of Science Citations: 0

Abstract

We evaluated the use of MALDI-TOF MS for the identification of 3 major, dairy-associated Prototheca species, namely, Prototheca bovis (formerly P. zopfii genotype 2), P. blaschkeae, and P. ciferrii (formerly P. zopfii genotype 1). The MALDI-TOF MS spectra established for those species were introduced into the reference spectra library of the Bruker Biotyper MALDI-TOF MS analysis software. Next, 31 Prototheca isolates from Holstein cows with mastitis, from herds located in the midwestern area of Sao Paulo State, Brazil, were subjected to MALDI-TOF MS profiling. MALDI-TOF MS allowed identification of 22 of 27 P. bovis and 3 of 4 P. blaschkeae isolates with scores >2.0, with 5 of 27 P. bovis and 1 of 4 P. blaschkeae isolates identified only to the genus level. With our extended algae database, MALDI-TOF MS can contribute to quick and effective speciation of Prototheca from mastitis cases. (AU)

• FAPESP's process: 17/50461-5 - Bovine mastitis: control by local use of polyplex nanoparticles
• Grantee: Marcos Veiga dos Santos
• Support Opportunities: Regular Research Grants