INitial Steps of Insulin Action in Parotid Glands of Male Wistar Rats

Author(s)

DeVito-Moraes, Andre Guaraci ^{[1, 2, 3]} ; Marques, Victor Di Donato ^[2] ; Caperuto, Luciana Chagas ^{[2, 4]} ; Ibuki, Flavia Kazue ^[1] ; Nogueira, Fernando Neves ^[1] ; Francci, Carlos Eduardo ^[1] ; de Oliveira Carvalho, Carla Roberta ^[2]

Affiliation

^[1] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Av Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Av Prof Lineu Prestes 1524, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Nove Julho UNINOVE, Sch Dent, Discipline Dent Biomat, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[4] Fed Univ Sao Paulo UNIFESP, Dept Biol Sci, R Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

• Document type: Journal article
• Source: Cell Biochemistry and Biophysics; AUG 2021.
• Web of Science Citations: 0

Abstract

The parotid gland is the largest salivary gland. It produces watery saliva, rich in proteins (amylase, lysozymes, and antibodies). Due to the gland's morphological cytoarchitecture composed of only serous acini, it contributes almost 50% of total salivary volume upon stimulation. It has been reported that the prevalence of saliva secretion impairments, periodontitis, delayed wound healing, and xerostomia increase in diabetic patients. Herein we evaluated the acute effects of insulin on insulin receptor phosphorylation status and its substrates IRS-1 and IRS-2 in the parotid glands of adult male Wistar rats, using Western blot analyses. We confirmed an acute effect of insulin on IR/IRS/PI3K/Akt and MAPK intracellular pathway activation in the parotid glands of male Wistar rats similar to the classical metabolic targets of the hormone, like the liver. (AU)