Effect of LPT on lipid accumulation and calmodulin concentration of STZ-induced diabitic rats

• Grant number: 12/02739-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date until: July 01, 2012
• End date until: September 30, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Alyne Simões Gonçalves
• Grantee: Juliana Rodrigues de Castro
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 12/20845-2 - Effect of laser on calmodulin concentration of parotid glands of diabetic rats, BE.EP.IC

Abstract

Diabetes mellitus causes a series of biochemical and histological changes in the salivary glands of streptozotocin-induced diabetic rats. Furthermore, our group has investigated the effect of laser irradiation on salivary glands and our data demonstrated that this therapy would be helpful in cases of hypofunction of the salivary glands and xerostomia. However, the action mechanism of this therapy on the glandular tissue is unclear. In this sense, continuing our studies, the present study aims at the effect of laser irradiation on lipid accumulation and on calmodulin concentration, which are related to glandular hypofunction observed in diabetic animals. For this purpose, 78 Wistar rats will be divided into six groups: C0 (control) : simulation of irradiation, C5 : 5 J/cm2, C20: 20 J /cm2, D0 (diabetic) simulation of irradiation, D5:5 J/cm2, D20 : 20 J/cm2. The diode laser (660 nm), 100 mW of power, will be used in areas corresponding to the parotid and submandibular glands. The rats will be euthanized 24 h after irradiation and salivary glands removed for biochemical (calmodulin concentration) and histological (lipid accumulation by electron microscopy) analysis.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CASTRO, JULIANA R.; DE SOUZA, EUGEN M. N.; PARK, YUN-JONG; DE CAMPOS, LUANA; CHA, SEUNGHEE; ARANA-CHAVEZ, VICTOR E.; NICOLAU, JOSE; SIMOES, ALYNE. Low-power laser irradiation decreases lipid droplet accumulation in the parotid glands of diabetic rats. Journal of Biophotonics, v. 11, n. 4, APR 2018. (12/20845-2, 11/14013-1, 12/02739-0)