| Full text | |
| Author(s): |
Palermo, Gisele Cristina de Lima
[1, 2]
;
Coutoune, Natalia
[1, 2]
;
Bueno, Joao Gabriel Ribeiro
[1, 2]
;
Maciel, Lucas Ferreira
[1]
;
dosSantos, Leandro Vieira
[1, 2]
Total Authors: 5
|
| Affiliation: | [1] CNPEM, Brazilian Biorenewable Natl Lab, Brazilian Ctr Res Energy & Mat, BR-13083100 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Genet & Mol Biol Grad Program, Campinas, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | MICROBIAL BIOTECHNOLOGY; v. 14, n. 5 JUL 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
The development of high-performance xylose-fermenting yeast is essential to achieve feasible conversion of biomass-derived sugars in lignocellulose-based biorefineries. However, engineered C5-strains of Saccharomyces cerevisiae still present low xylose consumption rates under anaerobic conditions. Here, we explore alternative metabolisms involved in metal homeostasis, which positively affect C5 fermentation and analyse the non-obvious regulatory network connection of both metabolisms using time-course transcriptome analysis. Our results indicated the vacuolar Fe2+/Mn2+ transporter CCC1, and the protein involved in heavy metal ion homeostasis BSD2, as promising new targets for rational metabolic engineering strategies, enhancing xylose consumption in nine and 2.3-fold compared with control. Notably, intracellular metal concentration levels were affected differently by mutations and the results were compared with positive controls isu1 Delta, a Fe-S cluster scaffold protein, and ssk2 Delta, a component of MAPKKK pathway. Temporal expression profiles indicate a metabolic remodelling in response to xylose, demonstrating changes in the main sugar sensing signalling pathways. (AU) | |
| FAPESP's process: | 20/07918-7 - An integrated system approach to engineer yeast as a cell factory for lignocellulose-based biorefineries |
| Grantee: | Leandro Vieira dos Santos |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Regular Program Grants |
| FAPESP's process: | 18/00888-5 - Genetic engineering of Saccharomyces cerevisiae for xylose and glucose co-fermentation and second-generation ethanol production |
| Grantee: | João Gabriel Ribeiro Bueno |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/06254-8 - A stressful place for live: a deep understanding of selective pressures on yeast during alcoholic fermentations |
| Grantee: | Mirta Natalia Coutouné |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/08519-6 - Development of a genomic atlas for rational engineering of Saccharomyces cerevisiae aiming second-generation ethanol production |
| Grantee: | Leandro Vieira dos Santos |
| Support Opportunities: | Regular Research Grants |