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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MPDH forms the cytoophidium in zebrafis

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Author(s):
Keppeke, Gerson Dierley [1, 2] ; Chang, Chia-Chun [1] ; Antos, Christopher L. [1] ; Peng, Min [3] ; Sung, Li-Ying [3, 4] ; Andrade, Luis Eduardo Coelho [2] ; Liu, Ji-Long [1]
Total Authors: 7
Affiliation:
[1] ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210 - Peoples R China
[2] Univ Fed Sao Paulo, Div Rheumatol, Escola Paulista Med, BR-04023062 Sao Paulo, SP - Brazil
[3] Natl Taiwan Univ, Inst Biotechnol, Taipei 106 - Taiwan
[4] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115 - Taiwan
Total Affiliations: 4
Document type: Journal article
Source: Developmental Biology; v. 478, p. 89-101, OCT 2021.
Web of Science Citations: 1
Abstract

Inosine monophosphate dehydrogenase (IMPDH) catalyzes the rate-limiting step in de novo guanine nucleotide biosynthesis. Its activity is negatively regulated by the binding of GTP. IMPDH can form a membraneless subcellular structure termed the cytoophidium in response to certain changes in the metabolic status of the cell. The polymeric form of IMPDH, which is the subunit of the cytoophidium, has been shown to be more resistant to the inhibition by GTP at physiological concentrations, implying a functional correlation between cytoophidium formation and the upregulation of GTP biosynthesis. Herein we demonstrate that zebrafish IMPDH1b and IMPDH2 isoforms can assemble abundant cytoophidium in most of cultured cells under stimuli, while zebrafish IMPDH1a shows distinctive properties of forming the cytoophidium in different cell types. Point mutations that disrupt cytoophidium structure in mammalian models also prevent the aggregation of zebrafish IMPDHs. In addition, we discover the presence of the IMPDH cytoophidium in various tissues of larval and adult fish under normal growth conditions. Our results reveal that polymerization and cytoophidium assembly of IMPDH can be a regulatory machinery conserved among vertebrates, and with specific physiological purposes. (AU)

FAPESP's process: 17/20745-1 - Autoantibodies depletion on autoreactive plasmocytes by genome editing with the CRISPR/Cas9 system
Grantee:Gerson Dierley Keppeke
Support Opportunities: Research Grants - Young Investigators Grants