Use of beeswax as an alternative binder in the development of composite electrodes: an approach for determination of hydrogen peroxide in honey samples

Author(s)

de Oliveira, Paulo Roberto ^[1] ; Kalinke, Cristiane ^[2] ; Bonacin, Juliano Alves ^[2] ; Marcolino-Junior, Luiz Humberto ^[3] ; Bergamini, Marcio Fernando ^[3] ; Malaspina, Osmar ^[4] ; Ferreira Nocelli, Roberta Cornelio ^[1] ; Janegitz, Bruno Campos ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, BR-13600970 Araras, SP - Brazil

^[2] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

^[3] Univ Fed Parana, Dept Chem, BR-81531980 Curitiba, Parana - Brazil

^[4] Sao Paulo State Univ, Ctr Social Insects Studies, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: Electrochimica Acta; v. 390, SEP 10 2021.
• Web of Science Citations: 0

Abstract

The present paper describes a new way to construct composite electrodes by using beeswax as an alternative binder and graphite powder as the conductive phase. Beeswax can be an attractive binder since it is a biodegradable and renewable material. Also, it attributes to the composite material a good rigidity after dry at room temperature. The monitoring of hydrogen peroxide (H2O2) in honey is extremely important due to its antibacterial properties, which are vital for the maintenance of the hive. The determination of H2O2 by electrochemical techniques is widely noted in the literature, mainly using redox mediators or catalysts, such as Prussian blue (PB). Therefore, the proposed composite electrode was prepared with beeswax and graphite in n-hexane, and the PB was electrochemically synthesized by cyclic voltammetry. The constructed electrode showed a low resistance of electronic transfer (60 Omega), high electroactive surface area, and high electronic transference constant (1.73 x10(-3) cm s(-1)) when compared with other composite electrodes. The presence of PB on composite electrode allowed the amperometric determination of H2O2 with good linearity (R-2 = 0.997) at a concentration range of 1.0-180 mu mol L-1 H2O2, showing a sensitivity of 0.184 mu AL mu mol(-1). Consequently, it was possible to determine H2O2 accurately at three concentration levels in wild honey samples (RSD = 98.5-102%), suggesting the use of this alternative method for the quality control analysis of honey. (C) 2021 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 17/21097-3 - Bee-agriculture interactions: perspectives to sustainable use
• Grantee: Osmar Malaspina
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants