| Full text | |
| Author(s): |
de Araujo, Patricia Rocha
[1]
;
Fioramonti Calixto, Giovana Maria
[1, 2]
;
Sousa Araujo, Victor Hugo
[1]
;
Sato, Mariana Rillo
[1]
;
Rodero, Camila Fernanda
[1]
;
Oshiro-Junior, Joao Augusto
[3]
;
Bauab, Tais Maria
[1]
;
Chorilli, Marlus
[1]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Biosci, BR-13414903 Piracicaba, SP - Brazil
[3] Paraiba State Univ, BR-58429500 Campina Grande, Paraiba - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Medical Mycology; v. 59, n. 8, p. 821-827, AUG 2021. |
| Web of Science Citations: | 2 |
| Abstract | |
The present study reports the performance of the pigment hypericin (HYP)-loaded poloxamer-based mucoadhesive in situ gelling liquid crystalline precursor system (LCPS) for the treatment of vulvovaginal candidiasis (VVC) in mice. LCPS composed of 40% of ethoxylated and propoxylated cetyl alcohol, 30% of oleic acid and cholesterol (7:1), 30% of a dispersion of 16% poloxamer 407 and 0.05% of HYP (HYP-LCPS) was prepared and characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS) and ex vivo permeation and retention studies across vaginal porcine mucosa were performed. In addition, the antifungal properties of the HYP-LCPS were evaluated in a murine in vivo model; for this, infected C57BL female mice groups were treated with both HYP in solution and HYP-LCPS, and after 6 days colony forming unit (CFU)/ml count was performed. PLM and SAXS confirmed that HYP-LCPS is a microemulsion situated in boundary transition region confirming its action as an LCPS. When in contact with simulated vaginal fluid, HYP-LCPS became rigid and exhibited maltase crosses and bragg peaks characteristics of lamellar phase. Ex vivo permeation and retention studies showed that HYP-LCPS provides a localized treatment on the superficial layers of porcine vaginal mucosa. HYP-LCPS induced a significant reduction in the number of CFU/ml in the mice; thus this formulation indicated it is as effective as a commercial dosage form. It was concluded that LCPS maintains the biological activity of HYP and provides an adequate drug delivery system for this lipophilic molecule at the vaginal mucosa, being a promising option in cases of VVC. (AU) | |
| FAPESP's process: | 19/10261-2 - Evaluation of the potential of nanostructured lipid carriers for co-encapsulation of curcumin and fluconazole dispersed in thermally-responsive hydrogels in the treatment of vulvovaginal candidiasis |
| Grantee: | Victor Hugo Sousa Araujo |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/11198-4 - Nanostructured lipid carriers dispersed in situ gelling hydrogel for vaginal administration of hypericin associated with photodynamic therapy in the treatment of vulvovaginal candidiasis |
| Grantee: | Mariana Rillo Sato |
| Support Opportunities: | Scholarships in Brazil - Doctorate |