Interaction of fibroblasts and induced pluripotent stem cells with poly(vinyl alcohol)-based hydrogel substrates

Author(s)

Sanguinet, Eduardo de O. ^[1] ; Siqueira, Nataly M. ^{[2, 3]} ; Menezes, Felipe de C. ^[2] ; Rasia, Gisele M. ^[4] ; Lothhammer, Nivia ^[1] ; Soares, Rosane M. D. ^[2] ; Meirelles, Flavio V. ^[5] ; Bressan, Fabiana F. ^[5] ; Bos-Mikich, Adriana ^[1]

Affiliation

^[1] Univ Fed Rio Grande do Sul, ICBS, Dept Morphol Sci, BR-90040060 Porto Alegre, RS - Brazil

^[2] Univ Fed Rio Grande do Sul, Inst Chem, Dept Organ Chem, Porto Alegre, RS - Brazil

^[3] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5G IG6 - Canada

^[4] Univ Fed Rio Grande do Sul, Postgrad Program Mat Sci, Porto Alegre, RS - Brazil

^[5] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, FZEA USP, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 108, n. 3, p. 857-867, APR 2020.
• Web of Science Citations: 0

Abstract

Induced pluripotent stem cells (iPSCs) provide a promising means of creating custom-tailored cell lines for cellular therapies. Their application in regenerative medicine, however, depends on the possibility that the maintenance and differentiation of cells and organs occur under defined conditions. One major component of stem cell culture systems is the substrate, where the cells must attach and proliferate. The present study aimed to investigate the putative cytotoxic effects of poly(vinyl alcohol) (PVA)-based matrices on the in vitro culture of mouse fetal fibroblasts. In addition, the PVA-based hydrogels were used to determine the capacity of bovine induced pluripotent stem cells (biPSCs) to adhere and proliferate on synthetic substrates. Our results show that both cell types interacted with the substrate and presented proliferation during culture. The biPSCs formed new colonies when cell suspensions were placed onto the hydrogel surface for culture. These results may represent a new characterized xeno-free clinical grade culture system to be widely applied in cell-based therapies. (AU)

• FAPESP's process: 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach
• Grantee: Fabiana Fernandes Bressan
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC