| Full text | |
| Author(s): |
Marques, Michele Flavia Sousa
[1, 2]
;
de Moura, Guilherme Santana
[1]
;
de Souza, Fernando Nogueira
[3, 4]
;
Gargano, Ronaldo Gomes
[4]
;
Santos, Kamila Reis
[4]
;
Ferronatto, Jose Augusto
[4]
;
Della Libera, Alice Maria Melville Paiva
[4]
;
Mota, Rinaldo Aparecido
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Fed Rural Pernambuco UFRPE, Dept Med Vet, Lab Doencas Infecciosas, Av Dom Manoel Medeiros S-N, BR-52171900 Recife, PE - Brazil
[2] Univ Fed Paraiba, Lab Caprinocultura & Ovinocultura, Ctr Ciencias Humanas Sociais & Agr, BR-58220000 Bananeiras, Paraiba - Brazil
[3] Univ Fed Paraiba, Programa Posgrad Ciencia Anim, BR-58397000 Areia - Brazil
[4] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, Av Prof Dr Orlando Marques Paiva 87, BR-05508270 Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Small Ruminant Research; v. 201, AUG 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
In this study, we evaluated the viability, intracellular production of reactive oxygen species (ROS) and phagocytosis of Staphylococcus aureus by blood polymorphonuclear leukocytes (PMNLs) in primiparous and pluriparous Santa Ine circumflex accent s and Lacaune ewes. Blood was collected from 14 Santa Ine circumflex accent s ewes (seven primiparous and seven pluriparous) and 14 Lacaune ewes (seven primiparous and seven pluriparous). The viability of PMNLs, intracellular ROS production, and S. aureus phagocytosis were evaluated using flow cytometry. No parity effect on blood's PMNLs functionality was found. However, comparing primiparous and pluriparous ewes, we noticed a difference in the opsonized phagocytic response to S. aureus, suggesting that pluriparous ewes could be better protected against S. aureus, although further field studies are needed. Antibody-mediated opsonization of S. aureus was associated with a lower intensity of S. aureus phagocytosis in both primiparous and pluriparous ewes, while it had no effect on the percentage of S. aureus phagocytosis. The percentage and geometric mean fluorescence intensity (GMFI) of S. aureus phagocytosis behaved differently in Lacaune and Santa Ine circumflex accent s ewes, which could be explained by breed and farm effects. The intensity of intracellular ROS production by PMNLs when stimulated with S. aureus was higher than that of unstimulated basal PMNLs in both primiparous and pluriparous ewes, while no effect on the percentage of PMNLs that produced ROS was observed. Thus, our study provides novel insights into PMNLs' functionality in primiparous and pluriparous ewes, which could impact their health, although further research is needed to extrapolate conclusions to the field. (AU) | |
| FAPESP's process: | 14/23189-4 - Intramammary infections by Staphylococcus aureus: crosstalk between mammary epithelial cells and peripheral blood mononuclear cells, and how it governs immunity in the mammary gland of dairy cows |
| Grantee: | Fernando Nogueira de Souza |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/03685-8 - Impact of teat channel occlusion on the metagenomic profile and its reflex in health of the bovine mammary gland |
| Grantee: | José Augusto Ferronatto |
| Support Opportunities: | Scholarships in Brazil - Master |