Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High-solid enzymatic hydrolysis of sugarcane bagasse and ethanol production in repeated batch process using column reactors

Full text
Author(s):
Ramos, Lucas [1] ; Vasconcelos, Marcelo H. [1, 2] ; Milagres, Adriane M. F. [1] ; Ferraz, Andre [1] ; Dias, Marina O. S. [3] ; Mendes, Fernanda M. [4] ; dos Santos, Julio C. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Dept Biotecnol, Escola Engn Lorena, BR-12602810 Sao Paulo - Brazil
[2] Inst Fed Educ Ciencia & Tecnol Rondonia IFRO, Campus Guajara Mirim, BR-76850000 Rondonia - Brazil
[3] Univ Fed Sao Paulo ICT UNIFESP, Inst Ciencia & Tecnol, BR-12247014 Sao Jose Dos Campos, SP - Brazil
[4] Ctr Nacl Pesquisa Energia & Mat CNPEM, Lab Nacl Biorenovaveis LNBR, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: 3 BIOTECH; v. 11, n. 10 OCT 2021.
Web of Science Citations: 0
Abstract

Alkaline sulfite pretreated sugarcane bagasse was enzymatically hydrolyzed in a packed-bed column reactor and a bubble column reactor was evaluated to produce ethanol from the hydrolysate. Initial solid loadings of 9-16% were used in column reactor in the hydrolysis step, and the use of lower value (9%) resulted in 41 g L-1 of glucose in the hydrolysate, corresponding to 87% of cellulose hydrolysis yield. This yield was reduced to 65% for a solid loading of 16%, corresponding to a glucose concentration of 54 g L-1. Subsequently, Saccharomyces cerevisiae and Scheffersomyces stipitis were used for ethanol production in medium based on hydrolysate previously obtained, using different aeration flowrates (0.3, 0.5 and 0.7 vvm). In simple batch fermentation using S. cerevisiae, higher ethanol yield (0.40 g.g(-1)) and productivity (1.58 g.L-1.h(-1)) were achieved using 0.5 vvm. When S. stipitis was used in simple batch co-fermentations, the maximum ethanol productivities were obtained using 0.5 and 0.7 vvm (0.64 and 0.63 g.L-1.h(-1), respectively). Successive repeated batches resulted in average ethanol concentration of 38 g.L-1 and fermentation efficiency of 82%, when using S. cerevisiae. For S. stipitis, those values were, respectively, 36 g.L-1 and 50%, with volumetric productivity increased along the cycles. Thus, the potential of the bioreactors as simple systems for use in the biological steps of biorefineries was demonstrated. (AU)

FAPESP's process: 15/10756-0 - Development of biorefinery models using experimental sugarcane hybrids and energy cane with different pretreatment methods
Grantee:Fernanda Machado Mendes Carvalho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
Grantee:Andre Luis Ferraz
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants