An appraisal of experimental designs: Application to enantioselective capillary electromigration techniques

Author(s)

Perovani, Icaro Salgado ^[1] ; Serpellone, Carolina Oliveira ^[1] ; de Oliveira, Anderson Rodrigo Moraes ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] UNESP, Natl Inst Alternat Technol Detect Toxicol Evaluat, Inst Chem, BR-14800900 Araraquara, SP - Brazil

• Document type: Review article
• Source: ELECTROPHORESIS; v. 42, n. 17-18, SI, p. 1726-1743, SEP 2021.
• Web of Science Citations: 0

Abstract

Enantioresolution processes are vital tools for investigating the enantioselectivities of chiral compounds. An analyst resolves to optimize enantioresolution conditions once they are determined. Generally, optimization is conducted by a one-factor-at-a-time (OFAT) approach. Although this approach may determine an adequate condition for the method, it does not often allow the estimation of the real optimum condition. Experimental designs are conducive for the optimization of enantioresolution methods via capillary electromigration techniques (CETs). They can efficiently extract information from the behavior of a method and enable the estimation of the real optimum condition. Furthermore, the application of the analytical quality by design (AQbD) approach to the development of CET-based enantioselective methods is a trend. This article (i) offers an overview of the application of experimental designs to the development of enantioselective methods from 2015 to mid-2020, (ii) reveals the experimental designs that are presently employed in CET-based enantioresolutions, and (iii) offers a critical point of view on how the different experimental designs can aid the optimization of enantioresolution processes by considering the method parameters. (AU)

• FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
• Grantee: Maria Valnice Boldrin
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/07534-4 - Development of chromatographic / electrophoretic methods to be further applied in in vitro enzymatic inhibition studies and prediction of drug interactions of chiral pesticides - Phase 2
• Grantee: Anderson Rodrigo Moraes de Oliveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/02139-0 - Risk assessment of the chiral pesticide prothioconazole in human models: in vitro-in vivo correlation, prediction of drug-pesticide interaction and cyto- and genotoxicity studies
• Grantee: Icaro Salgado Perovani
• Support Opportunities: Scholarships in Brazil - Doctorate