Antibiofilm activity of laser ablation with indocyanine green activated by different power laser parameters compared with photodynamic therapy on root canals infected with Enterococcus faecalis

Author(s)

Yamamoto, Larissa Yumi ^[1] ; Loureiro, Caroline ^[1] ; Angelo Cintra, Luciano Tavares ^[1] ; Leonardo, Renato de Toledo ^[2] ; Banci, Henrique Augusto ^[1] ; Fernandes Ribeiro, Ana Paula ^[1] ; Sivieri-Araujo, Gustavo ^[1] ; Jacinto, Rogerio de Castilho ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP Sch Dent, Dept Prevent & Restorat Dent, Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Restorat Dent, Sch Dent, Araraquara, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
• Web of Science Citations: 0

Abstract

Background: Antimicrobial photodynamic therapy (aPDT) is used as an adjunct to endodontic treatment to enhance microbial reduction in the root canal system. However, studies evaluating the impact of laser ablation with Indocyanine Green (ICG) are scarce. Thus, this in vitro study aimed to evaluate the efficiency of laser ablation with ICG using different laser parameters compared with aPDT using photosensitizer methylene blue (MB) and curcumin (CUR) on the reduction of E. faecalis biofilms on root canals. Methods: Forty-nine human premolars were used after biomechanical instrumentation for standardization. The root canals were contaminated with E. faecalis for 10 days to form biofilms, and divided into 7 groups (n = 7): 0.01% MB activated by red laser - MB+RL; 0.05% CUR activated by blue LED - CUR+BL; 0.05% ICG activated by infrared diode laser (2.5 W power, 30 ms interval, and 30 ms duration) - ICG+DL 2.5/30/30; 0.05% ICG activated by infrared diode laser (2.5 W power, 300 ms interval, and 100 ms duration) - ICG+DL 2.5/300/100; 0.05% ICG activated by infrared diode laser (3 W power, 300 ms interval, and 100 ms duration) - ICG+DL 3/ 300/100; sterile saline solution (negative control) - NC; and 2.5% NaOCl (positive control) - PC. Root canal sampling was performed prior to and immediately after the different treatment protocols. Data were submitted to One- and Two-Way ANOVA, followed by Student-Newman-Keuls test or Fisher LSD's test (alpha = 0.05). Results: All aPDT protocols promoted significant CFU reductions compared with the NC; the highest CFU reduction was observed for PC (p < 0.05). Among the protocols the highest CFU reduction was promoted by laser ablation with ICG+DL 3/300/100 (p < 0.05) except compared with aPDT using CUR+BL (p > 0.05). Conclusions: Laser ablation protocol using ICG+DL proved to be efficient in reducing E. faecalis biofilms, especially when activated at 3/300/100 configuration. (AU)

• FAPESP's process: 19/17916-4 - Evaluation of antimicrobial activity of different photodynamic therapy protocols in root canals infected by Enterococcus faecalis biofilm: an in vitro study
• Grantee: Larissa Yumi Yamamoto
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/18741-0 - Proteomic profile of bacteria from root canals of symptomatic or asymptomatic teeth with apical periodontitis
• Grantee: Rogerio de Castilho Jacinto
• Support Opportunities: Regular Research Grants