Active methane processing microbes and the disproportionate role of NC10 phylum in methane mitigation in Amazonian floodplains

Author(s):Show less -

Bento, Marilia de S. ^[1] ; Barros, Dayane J. ^[2] ; Araujo, Maria Gabriella da S. ^[3] ; Da Roz, Rafael ^[4] ; Carvalho, Glauber Altrao ^[5] ; do Carmo, Janaina B. ^{[4, 1]} ; Toppa, Rogerio H. ^[4] ; Neu, Vania ^[3] ; Forsberg, Bruce R. ^[6] ; Bodelier, Paul L. E. ^[7] ; Tsai, Siu M. ^[8] ; Navarrete, Acacio A. ^{[4, 1, 2, 9]}

Affiliation

^[1] Univ Fed Sao Carlos, Grad Program Biotechnol & Environm Monitoring, BR-18052780 Sorocaba, SP - Brazil

^[2] Fed Univ Tocantins, Grad Program Biodivers & Biotechnol, BR-77001090 Palmas, TO - Brazil

^[3] Fed Rural Univ Amazonia, Inst Socio Environm Aqua Resources, BR-66077530 Belem, PA - Brazil

^[4] Univ Fed Sao Carlos, Dept Environm Sci, BR-18052780 Sorocaba, SP - Brazil

^[5] Univ Fed Mato Grosso do Sul, Fac Engn Architecture & Urbanism, BR-79106550 Campo Grande, MS - Brazil

^[6] Vermont Dept Environm Conservat VTDEC VAEL, Randolph Ctr, Randolph, VT 05061 - USA

^[7] Netherlands Inst Ecol NIOO KNAW, Dept Microbial Ecol, NL-6708 PB Wageningen - Netherlands

^[8] Ctr Nucl Energy Agr, Cell & Mol Biol Lab, BR-13400970 Piracicaba, SP - Brazil

^[9] Univ Brasil, Grad Program Environm Sci, Estr Projetada F1, BR-15613899 Fernandopolis, SP - Brazil

• Document type: Journal article
• Source: BIOGEOCHEMISTRY; v. 156, n. 2 SEP 2021.
• Web of Science Citations: 0

Abstract

Here we use a top-down and bottom-up approach in landscape ecology to analyze the active microbes processing methane fluxes (FCH4) in seasonally flooded-forest (FOR) and -traditional farming systems (TFS) in Amazonian floodplains flooded with black, white, and clear water. Our results revealed higher CH4 emissions from water-atmosphere interface in clear water floodplain, followed by black and white water floodplain, respectively. Active methanogenic and methanotrophic taxa were ubiquitous at 0-15 and 15-30 cm soil layer in FOR and TFS, with differences among the water types with respect to the richness, evenness and diversity of the methanogenic communities. These ecological results were not generalizable regarding to FOR and TFS sites, soil layers, and non-flooded and flooded periods. Despite the predominant oxidation of CH4 in the non-flooded period, higher richness and diversity of methanotrophs were revealed for FOR and TFS in the flooded period. In turn, the structure of the methanogenic and methanotrophic communities and their variation were influenced mainly by soil physicochemical factors, water type, soil depth and the presence of nitrifiers, as Nitrososphaera and Nitrospira. Our study reveals a signature across methanotrophic communities in soils from Amazon floodplain with different water types, with a putative disproportionate role of NC10 phylum in CH4 mitigation in natural and agricultural Amazonian floodplains. These findings open the possibilities to explore the role of NC10 phylum in the carbon cycling in Amazon. (AU)

• FAPESP's process: 17/03575-5 - Dynamics of production and consumption of methane by active microbiota in Amazonian wetlands
• Grantee: Acacio Aparecido Navarrete
• Support type: Scholarships in Brazil - Research Program on Global Climate Change - Young Investigators
• FAPESP's process: 17/06415-9 - Dry and flood dynamics of methanothophs and methanogens on floodplains from the Negro and Solimões rivers in the Brazilian Amazon
• Grantee: Marília de Souza Bento
• Support type: Scholarships in Brazil - Master
• FAPESP's process: 18/02277-3 - High-throughput taxonomy annotation based on cDNA sequences of methanogenic and methanotrophic functional genes recovered from Amazonian wetlands
• Grantee: Marília de Souza Bento
• Support type: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 16/16687-3 - Dynamics of production and consumption of methane by active microbiota in Amazonian wetlands
• Grantee: Acacio Aparecido Navarrete
• Support type: Research Program on Global Climate Change - Young Investigators
• FAPESP's process: 18/15847-2 - Training in physicochemical analysis and determination of methane by gas chromatography from environmental samples from Amazonian wetlands
• Grantee: Larissa Fernandes Pereira
• Support type: Scholarships in Brazil - Technical Training Program - Technical Training