| Full text | |
| Author(s): |
Tanamachi, Amanda Rodrigues
[1, 2]
;
Fernandes, Fabio Henrique
[1, 2]
;
de Souza Vendemiatti, Josiane Aparecida
[3]
;
Prediger, Patricia
[3]
;
Camparotto, Natalia Gabriele
[3]
;
Rocha, Noeme Sousa
[4]
;
Umbuzeiro, Gisela Aragao
[3]
;
Favero Salvadori, Daisy Maria
[1, 2]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ Unesp, Med Sch, Dept Pathol, Botucatu, SP - Brazil
[2] Natl Inst Alternat Technol Detect Toxicol Evaluat, Inst Chem, Araraquara, SP - Brazil
[3] State Univ Campinas UNICAMP, Sch Technol, Limeira, SP - Brazil
[4] Sao Paulo State Univ UNESP, Sch Vet & Zootech, Botucatu, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Environmental and Molecular Mutagenesis; v. 62, n. 8 SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Dinitrophenylazo dyes can form 2-phenylbenzotriazoles (PBTAs) in the textile dyeing process upon the addition of chemical reducing agents. Some dinitrophenylazo dyes, as well as their respective reduced (non-chlorinated) and chlorinated PBTAs, are now found in rivers owing to wastewater from textile plants. This study aimed to investigate the genotoxicity of a new PBTA derived from C.I. Disperse Violet 93 azo dye, namely non-Cl PBTA-9. Primary DNA damage in the blood, liver, and colon cells, micronucleated cells in the bone marrow, and gene expression (NAT2, CYP1A1, TRP53, and CDKN1A) in liver cells were observed in mice, at acute oral exposure (gavage) doses of 5, 50, and 500 mu g/kg body weight (b.w.). The non-chlorinated PBTA-9 caused DNA damage in the blood and liver (at 500 mu g/kg b.w.) and in colon cells (at 5, 50, and 500 mu g/kg), and increased the frequency of micronucleated cells in the bone marrow (at 5 and 50 mu g/kg). No histological alterations or gene expression changes were observed. In conclusion, in vivo exposure to non-chlorinated PBTA-9 induced genetic damage in various rodent tissues, corroborating results previously obtained from the Ames test. Because this compound has been detected in rivers, exposure to humans and biota is a major concern. (AU) | |
| FAPESP's process: | 18/04105-5 - Toxicogenomic potential of environmental contaminant 2-phenylbenzotriazole 9 (PBTA-9) in vivo |
| Grantee: | Amanda Rodrigues Tanamachi |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 19/07822-2 - Synthesis of new nanoadsorbents, their characterizations and applications in pollutant adsorption in water |
| Grantee: | PATRICIA PREDIGER |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies |
| Grantee: | Maria Valnice Boldrin |
| Support Opportunities: | Research Projects - Thematic Grants |