MICA and KIR: Immunogenetic Factors Influencing Left Ventricular Systolic Dysfunction and Digestive Clinical Form of Chronic Chagas Disease

Author(s)

Ayo, Christiane Maria ^[1] ; Bestetti, Reinaldo Bulgarelli ^{[2, 3]} ; Junior, Eumildo de Campos ^[4] ; Ronchi, Luiz Sergio ^[4] ; Borim, Aldenis Albaneze ^[4] ; Brandao, Cinara Cassia ^[1] ; de Matttos, Luiz Carlos ^[1]

Affiliation

^[1] Med Sch Sao Jose Do Rio Preto, Mol Biol Dept, Immunogenet Lab, Sao Jose Do Rio Preto - Brazil

^[2] Univ Ribeirao Preto, Dept Med, Ribeirao Preto - Brazil

^[3] Med Sch Sao Jose Do Rio Preto, Dept Cardiol & Cardiovasc Surg, Sao Jose Do Rio Preto - Brazil

^[4] Med Sch Sao Jose Do Rio Preto, Surg Dept, Sao Jose Do Rio Preto - Brazil

• Document type: Journal article
• Source: FRONTIERS IN IMMUNOLOGY; v. 12, AUG 13 2021.
• Web of Science Citations: 0

Abstract

Tissue damage observed in the clinical forms of chronic symptomatic Chagas disease seems to have a close relationship with the intensity of the inflammatory process. The objective of this study was to investigate whether the MICA (MHC class I-related chain A) and KIR (killer cell immunoglobulin-like receptors) polymorphisms are associated with the cardiac and digestive clinical forms of chronic Chagas disease. Possible influence of these genes polymorphisms on the left ventricular systolic dysfunction (LVSD) in patients with chronic Chagas heart disease was also evaluated. This study enrolled 185 patients with positive serology for Trypanosoma cruzi classified according to the clinical form of the disease: cardiac (n=107) and digestive (n=78). Subsequently, patients with the cardiac form of the disease were sub-classified as with LVSD (n=52) and without LVSD (n=55). A control group was formed of 110 healthy individuals. Genotyping was performed by polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP). Statistical analyzes were carried out using the Chi-square test and odds ratio with 95% confidence interval was also calculated to evaluate the risk association. MICA-129 allele with high affinity for the NKG2D receptor was associated to the LVSD in patients with CCHD. The haplotype MICA{*}008\~{}HLA-C{*}06 and the KIR2DS2(-)/KIR2DL2(-)/KIR2DL3(+)/C1(+) combination were associated to the digestive clinical form of the disease. Our data showed that the MICA and KIR polymorphisms may exert a role in the LVSD of cardiac patients, and in digestive form of Chagas disease.</p> (AU)

• FAPESP's process: 13/06580-9 - KIR genes, their HLA ligands and MICA gene polymorphism in ocular toxoplasmosis and in digestive form of Chagas disease
• Grantee: Christiane Maria Ayo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/08075-4 - Immunogenetic markers related to Chagas disease
• Grantee: Luiz Carlos de Mattos
• Support Opportunities: Regular Research Grants