Advanced search
Start date
Betweenand

KIR genes, their HLA ligands and MICA gene polymorphism in ocular toxoplasmosis and in digestive form of Chagas disease

Grant number: 13/06580-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2013
Effective date (End): June 30, 2017
Field of knowledge:Health Sciences - Collective Health
Principal Investigator:Luiz Carlos de Mattos
Grantee:Christiane Maria Ayo
Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The toxoplasmosis and Chagas disease are caused by parasitic protozoa T.gondii and T. cruzi, respectively. While toxoplasmosis is clinically manifested in the forms of the eye, brain and congenital Chagas disease after a period of approximately two decades without symptoms, manifests itself in chronic one-third of individuals. The clinical manifestations of this disease include chronic irreversible lesions in some organs and approximately 30% of patients develop chronic Chagas heart disease and 10% in the gastrointestinal tract (magaesôfago and / or megacolon). The progression of an infection and the development of different clinical forms and different degrees of severity, can be related to the genetic characteristics of the pathogen and the host. Among the host factors, the immune response arouses special interest, and the genetic markers play an important modulatory role in this context, since several studies have reported the importance of immune response genes in resistance or susceptibility to disease. The overall goal of this project is to test the hypothesis that genes KIR (killer cell immunoglobulin-like receptors) and MICA (major histocompatibility complex class I-related chain A) are associated with ocular toxoplasmosis and digestive form of Chagas disease. Specific objectives include: 1. Select two groups of patients with toxoplasmosis Like this: TO-1 Group - exudative lesion and / or scar corioretiniana; Group TO-2 - no exudative lesions and / or scar corioretiana, and select two groups of patients with disease Chronic Chagas Like this: CH-1 group - patients with gastrointestinal clinical form of Chagas disease (megaesophagus and megacolon), Group-CH 2 - individuals with the cardiac form, mixed or asymptomatic with positive serology for Chagas disease. 2nd. Verifying the presence of anti-T. gondii and T. cruzi IgM and IgG in the serum of these patients, as indicators of acute or chronic infection, 3. Identifying the KIR genes, HLA class I (HLA-A, HLA-B and HLA-C), binders KIR, and MICA gene polymorphism in all groups of patients, 4. Checking whether these genes are associated with the development of ocular toxoplasmosis and digestive form of Chagas disease. Results will be analyzed using the Arlequin program version 3.1 to determine the Hardy-Weinberg equilibrium and the allele frequencies of HLA gene and haplotype and MICA. The KIR gene frequency is obtained by direct counting. The comparison between the groups will be done in 2x2 contingency table using the chi-square test with Yates correction or Fisher exact test. Odds ratio and confidence interval of 95% are calculated to verify the estimates of association using the program OpenEpi version 2.3.1. Our hypothesis is that MICA and KIR genes are associated with ocular toxoplasmosis and digestive form of Chagas disease. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DO CARMO CORREA, DEBORAH ELZITA; AYO, CHRISTIANE MARIA; LAGUILA VISENTAINER, JEANE ELIETE; AMBROSIO-ALBUQUERQUE, ELIANE PAPA; REIS, PAMELA GUIMARAES; BRANDAO DE MATTOS, CINARA CASSIA; BESTETTI, REINALDO BULGARELLI; DE MATTOS, LUIZ CARLOS; SELL, ANA MARIA. Human platelet antigen polymorphisms and the risk of chronic Chagas disease cardiomyopathy. PLATELETS, SEP 2019. Web of Science Citations: 0.
REIS, PMELA GUIMARAES; AYO, CHRISTIANE MARIA; DE MATTOS, LUIZ CARLOS; BRANDAO DE MATTOS, CINARA DE CSSIA; SAKITA, KARINA MAYUMI; DE MORAES, AMARILIS GIARETTA; MULLER, LARISSA PIRES; AQUINO, JULIMARY SUEMATSU; MACEDO, LUCIANA CONCI; MAZINI, PRISCILA SAAMARA; SELL, ANA MARIA; DE OLIVEIRA MARQUES, DIVINA SEILA; BESTETTI, REINALDO BULGARELLI; LAGUILA VISENTAINER, JEANE ELIETE. Genetic Polymorphisms of IL17 and Chagas Disease in the South and Southeast of Brazil. JOURNAL OF IMMUNOLOGY RESEARCH, 2017. Web of Science Citations: 1.
AYO, CHRISTIANE MARIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; BRANDAO DE MATTOS, CINARA DE CASSIA; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; DE MATTOS, LUIZ CARLOS. Ocular toxoplasmosis: susceptibility in respect to the genes encoding the KIR receptors and their HLA class I ligands. SCIENTIFIC REPORTS, v. 6, NOV 9 2016. Web of Science Citations: 4.
DE OLIVEIRA, AMANDA P.; AYO, CHRISTIANE M.; BESTETTI, REINALDO B.; BRANDAO DE MATTOS, CINARA C.; CAVASINI, CARLOS E.; DE MATTOS, LUIZ C. The role of CCR5 in Chagas disease - a systematic review. INFECTION GENETICS AND EVOLUTION, v. 45, p. 132-137, NOV 2016. Web of Science Citations: 7.
AYO, CHRISTIANE MARIA; DA SILVEIRA CAMARGO, ANA VITORIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; BARBOSA, AMANDA PIRES; BRANDAO DE MATTOS, CINARA DE CASSIA; DE MATTOS, LUIZ CARLOS. MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis. PLoS One, v. 10, n. 12 DEC 16 2015. Web of Science Citations: 3.
AYO, CHRISTIANE MARIA; DE OLIVEIRA, AMANDA PRISCILA; DA SILVEIRA CAMARGO, ANA VITORIA; BRANDAO DE MATTOS, CINARA CASSIA; BESTETTI, REINALDO BULGARELLI; DE MATTOS, LUIZ CARLOS. Association of the Functional MICA-129 Polymorphism With the Severity of Chronic Chagas Heart Disease. Clinical Infectious Diseases, v. 61, n. 8, p. 1310-1313, OCT 15 2015. Web of Science Citations: 10.
AYO, C. M.; DA SILVEIRA CAMARGO, A. V.; XAVIER, D. H.; BATISTA, M. F.; CARNEIRO, O. A.; BRANDAO DE MATTOS, C. C.; RICCI, JR., O.; DE MATTOS, L. C. Frequencies of allele groups HLA-A, HLA-B and HLA-DRB1 in a population from the northwestern region of Sao Paulo State, Brazil. INTERNATIONAL JOURNAL OF IMMUNOGENETICS, v. 42, n. 1, p. 19-25, FEB 2015. Web of Science Citations: 4.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.