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Class II HLA genes (DRB1 and DQB1) as risck factors for ocular toxoplasmosis

Grant number: 13/25650-8
Support type:Regular Research Grants
Duration: May 01, 2014 - April 30, 2016
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Luiz Carlos de Mattos
Grantee:Luiz Carlos de Mattos
Home Institution: Faculdade de Medicina de São José do Rio Preto (FAMERP). Secretaria de Desenvolvimento Econômico (São Paulo - Estado). São José do Rio Preto , SP, Brazil

Abstract

The toxoplasmosis, a disease resulting from infection with Toxoplasma gondii, is clinically manifested in ways ocular palsy and congenital. This pararsito Apicomplexa is capable of infecting cells of all nucleated tissues and may Remain in a latent or cause irreversible cell damage. The HLA class I and II control the adaptive immune responses and cellular and humoral influence susceptibility and resistance to infectious and parasitic diseases. The toxoplasmosis, besides being dependent on infection with T. gondii appears to be dependent on host genetic factors as well as the variability of the infecting strain. The aim of this project is to test the hypothesis that the HLA class II genes (HLA-DRB1 and HLA-DQB1) are associated with ocular toxoplasmosis. Specific objectives include: 1. Select two groups of patients thus formed: Group 1 (G1) - exudative lesion and / or scarring corioretiniana, Group 2 (G2) - absence of exudative lesions and / or scarring corioretiana; 2.Check the presence of anti-T . gondii IgM and IgG in the serum of these patients, as indicators of acute or chronic infection; 3. Identifying the HLA class II (HLA-DRB1 and the HLA-DQB1) in both groups; 4.Verificar one or more alleles of these genes are associated with toxoplasmosis. Will be analyzed at least 150 genomic DNA samples of patients regardless of ethnicity, consecutive, in the Outpatient Hospital Eye Base Foundation Regional Medical School of São José do Rio Preto, who had a clinical diagnosis of ocular toxoplasmosis. Will also be analyzed at least 150 genomic DNA samples of patients without a diagnosis of ocular toxoplasmosis. Genomic DNA extracted from white blood cells will be used for the identification of HLA class II using the PCR-SSO (Luminex). The identification of anti-T. gondii will be done with the use of ELISA and ELFA. An epidemiological data sheet will be completed for each patient. Both groups (G1 x G2) will be compared using the chi-square methods and / or Fisher's exact test, with a significance level of 5%. It will also be used to calculate ODDS RATIO. The frequencies of HLA class II will be evaluated to assess whether they are in accordance with the Hardy-Weinberg distribution, as recommended protocols for association study of genetic diseases. The results to be achieved with this project will be beneficial or not before the confirmation of the hypothesis being tested. In both situations, the results will know if the HLA class II genes are immunogenetic factors that influence the development of ocular toxoplasmosis. So, you can understand the importance of immunogenetic variation of individuals and populations for orientation programs to provide information, education, prevention and even treatment of ocular toxoplasmosis. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMILO, LILIAN MUNIZ; PEREIRA-CHIOCCOLA, VERA LUCIA; GAVA, RICARDO; MEIRA-STREJEVITCH, CRISTINA DA SILVA; VIDAL, JOSE ERNESTO; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; DE MATTOS, LUIZ CARLOS; JUNQUEIRA FRANCO SPEGIORIN, LIGIA COSENTINO; ANTUNES MURATA, FERNANDO HENRIQUE; FERREIRA, MARINA NEVES; ULISSES BARBOSA, DEUSENIA MACHADO; GONCALVES, FAUSTO DA SILVA; DIAS, CRISTIANE MORAES; CATELAN, MARCIA WAKAI; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; CAVALLINI, DANILO; GRP, FAMERP TOXOPLASMA RES. Molecular diagnosis of symptomatic toxoplasmosis: a 9-year retrospective and prospective study in a referral laboratory in Sao Paulo, Brazil. Brazilian Journal of Infectious Diseases, v. 21, n. 6, p. 638-647, NOV-DEC 2017. Web of Science Citations: 3.
MAIA, M. M.; MEIRA-STREJEVITCH, C. S.; PEREIRA-CHIOCCOLA, V. L.; DE HIPPOLITO, D. D. C.; SILVA, V. O.; BRANDAO DE MATTOS, C. C.; FREDERICO, F. B.; SIQUEIRA, R. C.; DE MATTOS, L. C.; GRP, FAMERP IAL TOXOPLASMA RES. Evaluation of gene expression levels for cytokines in ocular toxoplasmosis. PARASITE IMMUNOLOGY, v. 39, n. 10 OCT 2017. Web of Science Citations: 4.
MEIRA-STREJEVITCH, CRISTINA SILVA; PEREIRA-CHIOCCOLA, VERA LUCIA; MAIA, MARTA MARQUES; CARNIETTO DE HIPPOLITO, DAISE DAMARIS; WANG, HUI-TZU LIN; MOTOIE, GABRIELA; DE SOUZA GOMES, APARECIDA HELENA; KANAMURA, CRISTINA TAKAMI; MARTINES, ROOSECELIS BRASIL; BRANDAO DE MATTOS, CINARA CASSIA; FREDERICO, FABIO BATISTA; DE MATTOS, LUIZ CARLOS; GRP, FAMERP TOXOPLASMA RES. Reference genes for studies in infectious parasitic diseases in five types of human tissues. GENE REPORTS, v. 7, p. 98-105, JUN 2017. Web of Science Citations: 3.
AYO, CHRISTIANE MARIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; BRANDAO DE MATTOS, CINARA DE CASSIA; PREVIATO, MARIANA; BARBOSA, AMANDA PIRES; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; DE MATTOS, LUIZ CARLOS. Ocular toxoplasmosis: susceptibility in respect to the genes encoding the KIR receptors and their HLA class I ligands. SCIENTIFIC REPORTS, v. 6, NOV 9 2016. Web of Science Citations: 4.
AYO, CHRISTIANE MARIA; DA SILVEIRA CAMARGO, ANA VITORIA; FREDERICO, FABIO BATISTA; SIQUEIRA, RUBENS CAMARGO; PREVIATO, MARIANA; ANTUNES MURATA, FERNANDO HENRIQUE; SILVEIRA-CARVALHO, APARECIDA PERPETUO; BARBOSA, AMANDA PIRES; BRANDAO DE MATTOS, CINARA DE CASSIA; DE MATTOS, LUIZ CARLOS. MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis. PLoS One, v. 10, n. 12 DEC 16 2015. Web of Science Citations: 3.
AYO, CHRISTIANE MARIA; DE OLIVEIRA, AMANDA PRISCILA; DA SILVEIRA CAMARGO, ANA VITORIA; BRANDAO DE MATTOS, CINARA CASSIA; BESTETTI, REINALDO BULGARELLI; DE MATTOS, LUIZ CARLOS. Association of the Functional MICA-129 Polymorphism With the Severity of Chronic Chagas Heart Disease. Clinical Infectious Diseases, v. 61, n. 8, p. 1310-1313, OCT 15 2015. Web of Science Citations: 10.
AYO, C. M.; DA SILVEIRA CAMARGO, A. V.; XAVIER, D. H.; BATISTA, M. F.; CARNEIRO, O. A.; BRANDAO DE MATTOS, C. C.; RICCI, JR., O.; DE MATTOS, L. C. Frequencies of allele groups HLA-A, HLA-B and HLA-DRB1 in a population from the northwestern region of Sao Paulo State, Brazil. INTERNATIONAL JOURNAL OF IMMUNOGENETICS, v. 42, n. 1, p. 19-25, FEB 2015. Web of Science Citations: 4.

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