Effects of relative centrifugation force on L-PRF: An in vivo submandibular boney defect regeneration study

Author(s)

Tovar, Nick ^{[1, 2, 3]} ; Jalkh, Ernesto B. Benalcazar ^{[4, 1]} ; Ramalho, Ilana S. ^[4] ; Colon, Ricardo Rodriguez ^[5] ; Kim, Heoijin ^[1] ; Bonfante, Estevam A. ^[4] ; Torroni, Andrea ^[6] ; Coelho, Paulo G. ^{[6, 1, 7]} ; Witek, Lukasz ^{[8, 1]}

Affiliation

^[1] NYU, Coll Dent, Dept Biomat & Biomimet, 433 1st Ave, Room 842, New York, NY 10010 - USA

^[2] Bellevue Hosp Ctr, New York, NY 10016 - USA

^[3] NYU, Dept Oral & Maxillofacial Surg, Langone Med Ctr, New York, NY - USA

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Prosthodont & Periodontol, Bauru, SP - Brazil

^[5] Icahn Sch Med Mt Sinai, New York, NY 10029 - USA

^[6] NYU, Hansjorg Wyss Dept Plast Surg, Langone Med Ctr, New York, NY - USA

^[7] NYU, Tandon Sch Engn, Dept Mech & Aerosp Engn, Brooklyn, NY - USA

^[8] NYU, Tandon Sch Engn, Dept Biomed Engn, Brooklyn, NY - USA

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 109, n. 12, p. 2237-2245, DEC 2021.
• Web of Science Citations: 0

Abstract

Properties and composition of leukocyte- and platelet-rich fibrin (L-PRF) clots may be largely affected by centrifugation protocols (function of relative centrifugal force {[}RCF]), which may impact biological potential repair in bone regeneration. The present in vivo study sought to assess the effect of the RCF on the composition of L-PRF clots, as well as to compare the repair potential of L-PRF clots obtained with different RCF protocols in submandibular boney defects using PLGA scaffolds for bone regeneration. Complete blood count and volumetric evaluations were performed on L-PRF clots obtained through centrifugation for 12 min at 200, 400, and 600 RCF-clot centrifugation speeds. These evaluations were completed from blood collected immediately prior to any surgical procedures. The in vivo portion comprised of three submandibular unilateral, full thickness, osteotomies (similar to 0.40cm(3)) which were created in the submandibular region of six sheep, using rotary instrumentation under continuous irrigation. Subsequently, poly(lactic-co-glycolic acid) (PLGA) scaffolds were enveloped in a L-PRF membrane from one of the three spinning speeds (n = 6/RCF) and inserted into the defect (sites were interpolated to avoid site bias). Six-weeks after surgery, the mandibles were harvested en bloc and prepared for volumetric and histomorphometric evaluations. Membranes harvested from 600 RCF produced significantly larger L-PRF clots (6.97g +/- 0.95) in comparison to the lower 200 RCF (5.7g +/- 0.95), with no significant differences between 600 and 400, and from 400 and 200 RCF. The three tested RCFs did not alter the platelet count of the L-PRF clot. For the in vivo component, quantitative bone regeneration analyses demonstrated significantly higher values obtained with L-PRF membranes extracted post 600 RCF (27.01 +/- 8%) versus 200 RCF (17.54 +/- 8%), with no significant differences regarding 400 RCF (similar to 23 +/- 8%). At the qualitative histological analyses, L-PRF membranes obtained at 600 and 400 RCFs yielded improved healing throughout the defect, where the L-PRF sourced from the lowest speed, 200 RCF, presented healing primarily at the margins along with the presence of connective tissue at the central aspect of the surgical defect. Higher 600 RCF yielded larger L-PRF clots/membranes, resulting in enhanced bone repair potential in association with PLGA scaffolds for the treatment of critical size bone defects. (AU)

• FAPESP's process: 18/03072-6 - Experimental 70% Al2O3-30% ZrO2 composites: structural characterization and survival probability before and after aging
• Grantee: Ernesto Byron Benalcazar Jalkh
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/00452-5 - EXPERIMENTAL 70%Al2O3-30%ZrO2 ZTA COMPOSITES: MECHANICAL, ESTRUCTURAL AND TOPHOGRAPHICAL CHARACTERIZATION
• Grantee: Ernesto Byron Benalcazar Jalkh
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 12/19078-7 - Development and processing of Al2O3-ZrO2 translucent composite for CAD/CAM monolithic prostheses: the effect of aging on biaxial flexural strength
• Grantee: Estevam Augusto Bonfante
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 16/18818-8 - Multi-User Equipment approved in grant 2012/19078-7: ElectroPuls E3000 Linear-Torsion All-Electric dynamic test instrument package
• Grantee: Estevam Augusto Bonfante
• Support Opportunities: Multi-user Equipment Program