| Full text | |
| Author(s): |
Lima, Gabriela de Morais Gouvea
[1]
;
Borges, Aline Chiodi
[1]
;
Nishime, Thalita Mayumi Castaldelli
[2]
;
Santana-Melo, Gabriela de Fatima
[1]
;
Kostov, Konstantin Georgiev
[3]
;
Mayer, Marcia Pinto Alves
[4]
;
Koga-Ito, Cristiane Yumi
[1, 5]
Total Authors: 7
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Oral Biopathol Grad Program, BR-12245000 Sao Jose Dos Campos - Brazil
[2] Leibniz Inst Plasma Sci & Technol, Felix Hausdorff Sts 2, D-17489 Greifswald - Germany
[3] Sao Paulo State Univ UNESP, Fac Engn Guaratingueta, Dept Phys, BR-12516410 Guaratingueta - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-12247016 Sao Paulo - Brazil
[5] Sao Paulo State Univ UNESP, Sao Jose dos Campos Inst Sci & Technol, Dept Environm Engn, BR-12247016 Sao Paulo - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Molecules; v. 26, n. 18 SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once-Group P1 (n = 10); twice (Day 11 and 13)-Group P2 (n = 10); or not treated-Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications. (AU) | |
| FAPESP's process: | 16/07196-6 - Prospection of innovative therapeutic alternatives for infectious diseases with relevance in Dentistry: low temperature atmospheric pressure plasma and nanoencapsulate of bioactive substance |
| Grantee: | Cristiane Yumi Koga Ito |
| Support Opportunities: | Regular Research Grants |