Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease

Author(s)

Lima, Gabriela de Morais Gouvea ^[1] ; Borges, Aline Chiodi ^[1] ; Nishime, Thalita Mayumi Castaldelli ^[2] ; Santana-Melo, Gabriela de Fatima ^[1] ; Kostov, Konstantin Georgiev ^[3] ; Mayer, Marcia Pinto Alves ^[4] ; Koga-Ito, Cristiane Yumi ^{[1, 5]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Oral Biopathol Grad Program, BR-12245000 Sao Jose Dos Campos - Brazil

^[2] Leibniz Inst Plasma Sci & Technol, Felix Hausdorff Sts 2, D-17489 Greifswald - Germany

^[3] Sao Paulo State Univ UNESP, Fac Engn Guaratingueta, Dept Phys, BR-12516410 Guaratingueta - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-12247016 Sao Paulo - Brazil

^[5] Sao Paulo State Univ UNESP, Sao Jose dos Campos Inst Sci & Technol, Dept Environm Engn, BR-12247016 Sao Paulo - Brazil

• Document type: Journal article
• Source: Molecules; v. 26, n. 18 SEP 2021.
• Web of Science Citations: 0

Abstract

Due to the limitations of traditional periodontal therapies, and reported cold atmospheric plasma anti-inflammatory/antimicrobial activities, plasma could be an adjuvant therapy to periodontitis. Porphyromonas gingivalis was grown in blood agar. Standardized suspensions were plated on blood agar and plasma-treated for planktonic growth. For biofilm, dual-species Streptococcus gordonii + P. gingivalis biofilm grew for 48 h and then was plasma-treated. XTT assay and CFU counting were performed. Cytotoxicity was accessed immediately or after 24 h. Plasma was applied for 1, 3, 5 or 7 min. In vivo: Thirty C57BI/6 mice were subject to experimental periodontitis for 11 days. Immediately after ligature removal, animals were plasma-treated for 5 min once-Group P1 (n = 10); twice (Day 11 and 13)-Group P2 (n = 10); or not treated-Group S (n = 10). Mice were euthanized on day 15. Histological and microtomography analyses were performed. Significance level was 5%. Halo diameter increased proportionally to time of exposure contrary to CFU/mL counting. Mean/SD of fibroblasts viability did not vary among the groups. Plasma was able to inhibit P. gingivalis in planktonic culture and biofilm in a cell-safe manner. Moreover, plasma treatment in vivo, for 5 min, tends to improve periodontal tissue recovery, proportionally to the number of plasma applications. (AU)

• FAPESP's process: 16/07196-6 - Prospection of innovative therapeutic alternatives for infectious diseases with relevance in Dentistry: low temperature atmospheric pressure plasma and nanoencapsulate of bioactive substance
• Grantee: Cristiane Yumi Koga Ito
• Support Opportunities: Regular Research Grants