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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

On the Weak Binding and Spectroscopic Signature of SARS-CoV-2 nsp14 Interaction with RNA

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Hassan, Ayaz [1] ; Sedenho, Graziela C. [1] ; Vitale, Phelipe A. M. [2] ; Oliviera, Mona N. [2] ; Crespilho, Frank N. [1]
Total Authors: 5
[1] Univ Sao Paulo, Sao Carlos Inst Chem, Dept Phys Chem, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil
[2] Biolinker, Av Prof Lineu Prestes, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CHEMBIOCHEM; v. 22, n. 24 OCT 2021.
Web of Science Citations: 0

The SARS-CoV-2 non-structural protein 14 (nsp14), known as exoribonuclease is encoded from the large polyprotein of viral genome and is a major constituent of the transcription replication complex (TRC) machinery of the viral RNA synthesis. This protein is highly conserved among the coronaviruses and is a potential target for the development of a therapeutic drug. Here, we report the SARS-CoV-2 nsp14 expression, show its structural characterization, and ss-RNA exonuclease activity through vibrational and electronic spectroscopies. The deconvolution of amide-I band in the FTIR spectrum of the protein revealed a composition of 35 % alpha-helix and 25 % beta-sheets. The binding between protein and RNA is evidenced from the spectral changes in the amide-I region of the nsp14, showing protein conformational changes during the binding process. A value of 20.60 +/- 3.81 mol L-1 of the binding constant (K-D) is obtained for nsp14/RNA complex. The findings reported here can motivate further studies to develop structural models for better understanding the mechanism of exonuclease enzymes for correcting the viral genome and can help in the development of drugs against SARS-CoV-2. (AU)

FAPESP's process: 20/04796-8 - In situ and operando studies of metaloenzymes for energy conversion and fuel bioelectrosynthesis
Grantee:Graziela Cristina Sedenho
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/12053-8 - High performance electrodes applied in organic batteries and in biofuel cell
Grantee:Frank Nelson Crespilho
Support Opportunities: Regular Research Grants
FAPESP's process: 19/15333-1 - Bio-photo-electrochemical hybrid cells for solar energy conversion
Grantee:Frank Nelson Crespilho
Support Opportunities: Regular Research Grants