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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Platelet microparticles load a repertory of miRNAs programmed to drive osteogenic phenotype

Full text
Author(s):
Ferreira, Marcel Rodrigues [1] ; Zambuzzi, Willian Fernando [1]
Total Authors: 2
Affiliation:
[1] Savo Paulo State Univ UNESP, Inst Biosci, Dept Chem & Biochem, Campus Botucatu, BR-18618970 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 109, n. 8, p. 1502-1511, AUG 2021.
Web of Science Citations: 1
Abstract

Autologous platelet-rich plasma accelerates bone healing by releasing biomolecules during their degranulation process, which are transported by vesicle-like structures called platelet microparticles (PMPs). However, the underlying mechanisms regulating the osteogenic differentiation by PMP-released miRs remain poorly understood and this prompted us to better address this issue. Thus, miRNAseq expression profiles (E-GEOD-76789) were downloaded from ArrayExpress database. GEO2R was performed to evaluate the differential expression, and mirnatap R package was used to find targets for differentially expressed miRNAs. An extend protein-protein (ePPI) network for osteogenic marker proteins was generated using String, and DAVID tools were used to perform gene ontology and KEGG pathway analysis from ePPI and miRNAs targets. Our data show that ePPI network was composed by 232 nodes and 2,175 edges, with a clustering coefficient of 0.546. MCODE was able to identify seven clusters contained in the ePPI network, and the two that presented a score above 10 were used in further analysis. Conversely, 15,944 different targets were found as down-expressed while 5,715 different targets were up-expressed. Among the downregulated 75 miRNAs, 70 have predicted targets present in the ePPI network, while the 21 upregulated miRNAs have 19 predicted targets in the ePPI network. Our study provides a registry of miRNAs that play a central role in regulating osteogenic phenotype, which might have potential therapeutic applications in bone regeneration and bone tissue engineering. (AU)

FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 19/26854-2 - Effect of a hypoxia model on angiogenesis-osteogenesis coupling: a special look at micro vesicles and potential biotechnological applications
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Regular Research Grants
FAPESP's process: 18/05731-7 - Lyophilized PRP associated with nano-hydroxyapatite in cell performance and bone regeneration
Grantee:Marcel Rodrigues Ferreira
Support Opportunities: Scholarships in Brazil - Doctorate