Tracking the Evolutionary Trends Among Small-Size Fishes of the Genus Pyrrhulina (Characiforme, Lebiasinidae): New Insights From a Molecular Cytogenetic Perspective

Author(s):Show less -

Rosa de Moraes, Renata Luiza ^[1] ; Cavalcante Sassi, Francisco de Menezes ^[1] ; Carlos Bertollo, Luiz Antonio ^[1] ; Ferreira Marinho, Manoela Maria ^{[2, 3]} ; Viana, Patrik Ferreira ^[4] ; Feldberg, Eliana ^[4] ; Sales Oliveira, Vanessa Cristina ^[1] ; Deon, Geize Aparecida ^{[1, 5]} ; Al-Rikabi, Ahmed B. H. ^[6] ; Liehr, Thomas ^[6] ; Cioffi, Marcelo de Bello ^[1]

Affiliation

^[1] Univ Fed Sao Carlos UFSCar, Dept Genet & Evolucao, Lab Citogenet Peixes, Sao Carlos - Brazil

^[2] Univ Sao Paulo MZUSP, Museu Zool, Sao Paulo - Brazil

^[3] Univ Fed Paraiba UFPB, Dept Sistemat & Ecol DSE, Lab Sistemat & Morfol Peixes, Joao Pessoa, Paraiba - Brazil

^[4] Inst Nacl de Pesquisas da Amazonia, Lab Gentet Anim, Coordenacao Biodiversidade, Manaus, Amazonas - Brazil

^[5] Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Lab Biol Cromossom Estrutura & Funcao, Ponta Grossa, Parana - Brazil

^[6] Univ Hosp Jena, Inst Human Genet, Jena - Germany

• Document type: Journal article
• Source: FRONTIERS IN GENETICS; v. 12, OCT 6 2021.
• Web of Science Citations: 0

Abstract

Miniature fishes have always been a challenge for cytogenetic studies due to the difficulty in obtaining chromosomal preparations, making them virtually unexplored. An example of this scenario relies on members of the family Lebiasinidae which include miniature to medium-sized, poorly known species, until very recently. The present study is part of undergoing major cytogenetic advances seeking to elucidate the evolutionary history of lebiasinids. Aiming to examine the karyotype diversification more deeply in Pyrrhulina, here we combined classical and molecular cytogenetic analyses, including Giemsa staining, C-banding, repetitive DNA mapping, comparative genomic hybridization (CGH), and whole chromosome painting (WCP) to perform the first analyses in five Pyrrhulina species (Pyrrhulina aff. marilynae, Pyrrhulina sp., P. obermulleri, P. marilynae and Pyrrhulina cf. laeta). The diploid number (2n) ranged from 40 to 42 chromosomes among all analyzed species, but P. marilynae is strikingly differentiated by having 2n = 32 chromosomes and a karyotype composed of large meta/submetacentric chromosomes, whose plesiomorphic status is discussed. The distribution of microsatellites does not markedly differ among species, but the number and position of the rDNA sites underwent significant changes among them. Interspecific comparative genome hybridization (CGH) found a moderate divergence in the repetitive DNA content among the species' genomes. Noteworthy, the WCP reinforced our previous hypothesis on the origin of the X1X2Y multiple sex chromosome system in P. semifasciata. In summary, our data suggest that the karyotype differentiation in Pyrrhulina has been driven by major structural rearrangements, accompanied by high dynamics of repetitive DNAs.</p> (AU)

• FAPESP's process: 20/11772-8 - An integrative approach for the analysis of dispersal, adaptive and evolutionary processes between realted species with intercontinental distribution: the Osteoglossidae family (Teleostei, Osteoglossiformes) as an investigative model: part IV
• Grantee: Marcelo de Bello Cioffi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/02681-9 - Evolutionary relationships and demographic history in Loricariidae (Siluriformes) species, with emphasis on Harttia genus
• Grantee: Francisco de Menezes Cavalcante Sassi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/25045-3 - Chromosomal evolution, genetic diversity and demographic history in species of the genus Pyrrhulina (Characiformes, Lebiasinidae)
• Grantee: Renata Luiza Rosa de Moraes
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)