Genomic analysis of an outbreak of bovine tuberculosis in a man-made multi-host species system: A call for action on wildlife in Brazil

Author(s):Show less -

Lima, Daiane A. R. ^[1] ; Zimpel, Cristina K. ^{[2, 3]} ; Patane, Jose S. ^[4] ; Silva-Pereira, Taiana Taina ^{[2, 3]} ; Etges, Rodrigo N. ^[5] ; Rodrigues, Rudielle A. ^[1] ; Davila, Alberto M. R. ^{[6, 7]} ; Ikuta, Cassia Y. ^[2] ; Neto, Jose S. Ferreira ^[2] ; Guimaraes, Ana Marcia S. ^{[2, 3]} ; Araujo, Flabio R. ^[8]

Affiliation

^[1] Univ Fed Mato Grosso do Sul, Sch Vet Med, Dept Vet Med, Grad Program Vet Sci, Campo Grande, MS - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Lab Appl Res Mycobacteria, Sao Paulo, SP - Brazil

^[4] Ctr Bioinformat & Computat Biol, Butantan Inst, Sao Paulo, SP - Brazil

^[5] Secretary Agr, Livestock & Rural Dev, Porto Alegre, RS - Brazil

^[6] Oswaldo Cruz Inst, Computat & Syst Biol Lab, FIOCRUZ, Rio De Janeiro - Brazil

^[7] Oswaldo Cruz Inst, Grad Program Biodivers & Hlth, FIOCRUZ, Rio De Janeiro - Brazil

^[8] Embrapa Beef Cattle, Campo Grande, MS - Brazil

• Document type: Journal article
• Source: TRANSBOUNDARY AND EMERGING DISEASES; OCT 2021.
• Web of Science Citations: 0

Abstract

We report on a 15-year-long outbreak of bovine tuberculosis (bTB) in wildlife from a Brazilian safari park. A timeline of diagnostic events and whole-genome sequencing (WGS) of 21 Mycobacterium bovis isolates from deer and llamas were analyzed. Accordingly, from 2003 to 2018, at least 16 animals, from eight species, died due to TB, which is likely an underestimated number. In three occasions since 2013, the deer presented positive tuberculin tests, leading to the park closure and culling of all deer. WGS indicated that multiple M. bovis strains were circulating, with at least three founding introductions since the park inauguration in 1977. Using a previously sequenced dataset of 71 M. bovis genomes from cattle, we found no recent transmission events between nearby farms and the park based on WGS. Lastly, by discussing socio-economic and environmental factors escaping current regulatory gaps that were determinant of this outbreak, we pledge for the development of a plan to report and control bTB in wildlife in Brazil. (AU)

• FAPESP's process: 17/04617-3 - Host adaption of Mycobacterium tuberculosis and Mycobacterium bovis: a genomic and transcriptional approach
• Grantee: Cristina Kraemer Zimpel
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/10896-8 - Transcriptomic and metabolic features of Mycobacterium tuberculosis and Mycobacterium bovis during human macrophage infection
• Grantee: Cristina Kraemer Zimpel
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate