Insulin stimulates beta-alanine uptake in skeletal muscle cells in vitro

Author(s)

Santos, Livia ^[1] ; Goncalves, L. S. ^[2] ; Bagheri-Hanei, Shirin ^[3] ; Moller, Gabriella Berwig ^[2] ; Sale, Craig ^[1] ; James, Ruth M. ^[1] ; Artioli, Guilherme Giannini ^[4]

Affiliation

^[1] Nottingham Trent Univ, Musculoskeletal Physiol Res Grp, Sport Hlth & Performance Enhancement Res Ctr, Nottingham - England

^[2] Univ Sao Paulo, FMUSPEscola Educ Fis & Esporte, Fac Med, Appl Physiol & Nutr Res Grp, Rheumatol Div, Sao Paulo, SP - Brazil

^[3] Aston Univ, Coll Engn & Phys Sci, Birmingham B4 7ET, W Midlands - England

^[4] Manchester Metropolitan Univ, Dept Life Sci, John Dalton Bldg, Manchester M1 5GD, Lancs - England

• Document type: Journal article
• Source: Amino Acids; v. 53, n. 11 OCT 2021.
• Web of Science Citations: 0

Abstract

We evaluated whether insulin could stimulate beta-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with beta-alanine (350 or 700 mu mol.L-1), with insulin (100 mu U.mL(-1)) either added to the media or not. Insulin stimulated the beta-alanine uptake at the lower (350 mu mol.L-1) but not higher (700 mu mol.L-1) beta-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin. (AU)

• FAPESP's process: 14/11948-8 - Life without carnosine: development and characterization of a KO rat model for studying the physiological role of carnosine and its implications to physical exercise and muscle metabolism
• Grantee: Guilherme Giannini Artioli
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/25032-9 - The role of carnosine on Ca2+ handling, control of oxidative stress and protection against protein glycation: advances and applications of the study life without carnosine
• Grantee: Hamilton Augusto Roschel da Silva
• Support Opportunities: Regular Research Grants